ArticleGenetics2000
Comparative fluorescence in situ hybridization mapping of a 431-kb Arabidopsis thaliana bacterial artificial chromosome contig reveals the role of chromosomal duplications in the expansion of the Brassica rapa genome.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 92 citations in OpenAlex.
- Chromosome painting in plants: history and future perspectives.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Review
- Construction of cytogenetic map of Gossypium herbaceum chromosome 1 and its integration with genetic maps.Molecular cytogenetics · 2015Article
- FISH analysis of PTEN in endometrial carcinoma. Comparison with SNP arrays and MLPA.Histopathology · 2014Article
- Preparations of meiotic pachytene chromosomes and extended DNA fibers from cotton suitable for fluorescence in situ hybridization.PloS one · 2012Article
- Synteny between Brachypodium distachyon and Hordeum vulgare as revealed by FISH.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping.Chromosoma · 2010Article
- Genomic microstructure and differential expression of the genes encoding UDP-glucose:sinapate glucosyltransferase (UGT84A9) in oilseed rape (Brassica napus).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Sequenced BAC anchored reference genetic map that reconciles the ten individual chromosomes of Brassica rapa.BMC genomics · 2009Article
- Article
- Progress in understanding and sequencing the genome of Brassica rapa.International journal of plant genomics · 2008Article
- Article
- Predicting and testing physical locations of genetically mapped loci on tomato pachytene chromosome 1.Genetics · 2007Article
- Cytogenetics and genome analysis in Brassica crops.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
- A sequence-tagged linkage map of Brassica rapa.Genetics · 2006Article
- Article
- Comparative genomics reveals expansion of the FLC region in the genus Arabidopsis.Molecular genetics and genomics : MGG · 2006Article
- Physical mapping and microsynteny of Brassica rapa ssp. pekinensis genome corresponding to a 222 kbp gene-rich region of Arabidopsis chromosome 4 and partially duplicated on chromosome 5.Molecular genetics and genomics : MGG · 2005Article
- Molecular cytogenetic analysis of Brassica rapa-Brassica oleracea var. alboglabra monosomic addition lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Chromosome triplication found across the tribe Brassiceae.Genome research · 2005Article
- Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comparative genome studies are important contributors to our understanding of genome evolution. Most comparative genome studies in plants have been based on genetic mapping of homologous DNA loci in different genomes. Large-scale comparative physical mapping has been hindered by the lack of efficient and affordable techniques. We report here the adaptation of fluorescence in situ hybridization (FISH) techniques for comparative physical mapping between Arabidopsis thaliana and Brassica rapa. A set of six bacterial artificial chromosomes (BACs) representing a 431-kb contiguous region of chromosome 2 of A. thaliana was mapped on both chromosomes and DNA fibers of B. rapa. This DNA fragment has a single location in the A. thaliana genome, but hybridized to four to six B. rapa chromosomes, indicating multiple duplications in the B. rapa genome. The sizes of the fiber-FISH signals from the same BACs were not longer in B. rapa than those in A. thaliana, suggesting that this genomic region is duplicated but not expanded in the B. rapa genome. The comparative fiber-FISH mapping results support that chromosomal duplications, rather than regional expansion due to accumulation of repetitive sequences in the intergenic regions, played the major role in the evolution of the B. rapa genome.
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