ArticleGenetics2000
Respiratory chain complex I is essential for sexual development in neurospora and binding of iron sulfur clusters are required for enzyme assembly.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- The 24-kDa subunit of mitochondrial complex I regulates growth, microsclerotia development, stress tolerance, and virulence in Verticillium dahliae.BMC biology · 2024Article
- Article
- Dynamic Regulation of Peroxisomes and Mitochondria during Fungal Development.Journal of fungi (Basel, Switzerland) · 2020Review
- The mitochondrial complex I of trypanosomatids--an overview of current knowledge.Journal of bioenergetics and biomembranes · 2014Review
- New insights into the roles of NADPH oxidases in sexual development and ascospore germination in Sordaria macrospora.Genetics · 2014Article
- Novel insights into the role of Neurospora crassa NDUFAF2, an evolutionarily conserved mitochondrial complex I assembly factor.Molecular and cellular biology · 2013Article
- The reaction of NADPH with bovine mitochondrial NADH:ubiquinone oxidoreductase revisited: II. Comparison of the proposed working hypothesis with literature data.Journal of bioenergetics and biomembranes · 2010Article
- Metabolic and developmental effects resulting from deletion of the citA gene encoding citrate synthase in Aspergillus nidulans.Eukaryotic cell · 2010Article
- Eukaryotic complex I: functional diversity and experimental systems to unravel the assembly process.Molecular genetics and genomics : MGG · 2008Review
- Microarray analysis of transcript accumulation during perithecium development in the filamentous fungus Gibberella zeae (anamorph Fusarium graminearum).Molecular genetics and genomics : MGG · 2006Article
- Neurospora strains harboring mitochondrial disease-associated mutations in iron-sulfur subunits of complex I.Genetics · 2005Article
- The internal alternative NADH dehydrogenase of Neurospora crassa mitochondria.The Biochemical journal · 2003Article
- On complex I and other NADH:ubiquinone reductases of Neurospora crassa mitochondria.Journal of bioenergetics and biomembranes · 2001Review
Corrections and comments
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Authors and funding
2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We have cloned and disrupted in vivo, by repeat-induced point mutations, the nuclear gene coding for an iron sulfur subunit of complex I from Neurospora crassa, homologue of the mammalian TYKY protein. Analysis of the obtained mutant nuo21.3c revealed that complex I fails to assemble. The peripheral arm of the enzyme is disrupted while its membrane arm accumulates. Furthermore, mutated 21.3c-kD proteins, in which selected cysteine residues were substituted with alanines or serines, were expressed in mutant nuo21. 3c. The phenotypes of these strains regarding the formation of complex I are similar to that of the original mutant, indicating that binding of iron sulfur centers to protein subunits is a prerequisite for complex I assembly. Homozygous crosses of nuo21.3c strain, and of other complex I mutants, are unable to complete sexual development. The crosses are blocked at an early developmental stage, before fusion of the nuclei of opposite mating types. This phenotype can be rescued only by transformation with the intact gene. Our results suggest that this might be due to the compromised capacity of complex I-defective strains in energy production.
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