ArticleGenetics2000
Suppression of the profilin-deficient phenotype by the RHO2 signaling pathway in Saccharomyces cerevisiae.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Establishment of a yeast essential protein conditional-degradation library and screening for autophagy-regulating genes.Autophagy · 2025Article
- Profilin Pfy1 is critical for cell wall integrity and virulence inMicrobiology spectrum · 2025Article
- Sequential recruitment of F-BAR proteins controls cytoskeletal crosstalk at the yeast bud neck.Current biology : CB · 2025Article
- Quantitative analysis of septin Cdc10 & Cdc3-associated proteome during stress response in the fungal pathogen Cryptococcus neoformans.PloS one · 2024Article
- Transcriptomic Analysis Reveals That Rho GTPases Regulate Trap Development and Lifestyle Transition of the Nematode-Trapping FungusMicrobiology spectrum · 2022Article
- Common formin-regulating sequences in Smy1 and Bud14 are required for the control of actin cable assembly in vivo.Molecular biology of the cell · 2016Article
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- The F-BAR protein Syp1 negatively regulates WASp-Arp2/3 complex activity during endocytic patch formation.Current biology : CB · 2009Article
- Early-arriving Syp1p and Ede1p function in endocytic site placement and formation in budding yeast.Molecular biology of the cell · 2009Article
- Syp1 is a conserved endocytic adaptor that contains domains involved in cargo selection and membrane tubulation.The EMBO journal · 2009Article
- A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast.Genetics · 2008Article
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- Article
- Central roles of small GTPases in the development of cell polarity in yeast and beyond.Microbiology and molecular biology reviews : MMBR · 2007Review
- Motifs, themes and thematic maps of an integrated Saccharomyces cerevisiae interaction network.Journal of biology · 2005Article
- A role for GEA1 and GEA2 in the organization of the actin cytoskeleton in Saccharomyces cerevisiae.Genetics · 2003Article
- Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeast.The Journal of cell biology · 2003Article
- Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway.Genetics · 2002Article
- Saccharomyces cerevisiae Cdc42p localizes to cellular membranes and clusters at sites of polarized growth.Eukaryotic cell · 2002Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Profilin plays an important role in actin organization in all eukaryotic cells through mechanisms that are still poorly understood. We had previously shown that Mid2p, a transmembrane protein and a potential cell wall sensor, is an effective multicopy suppressor of the profilin-deficient phenotype in Saccharomyces cerevisiae. To better understand the role of Mid2p in the organization of the actin cytoskeleton, we isolated five additional multicopy suppressors of pfy1Delta cells that are Rom1p, Rom2p, Rho2p, Smy1p, and the previously uncharacterized protein Syp1p. The problems of caffeine and NaCl sensitivity, growth defects at 30 degrees and 37 degrees, the accumulation of intracellular vesicular structures, and a random budding pattern in pfy1Delta cells are corrected by all the suppressors tested. This is accompanied by a partial repolarization of the cortical actin patches without the formation of visible actin cables. The overexpression of Mid2p, Rom2p, and Syp1p, but not the overexpression of Rho2p and Smy1p, results in an abnormally thick cell wall in wild-type and pfy1Delta cells. Since none of the suppressors, except Rho2p, can correct the phenotype of the pfy1-111/rho2Delta strain, we propose a model in which the suppressors act through the Rho2p signaling pathway to repolarize cortical actin patches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.