ArticleGenetics2000
Suppressors of mdm20 in yeast identify new alleles of ACT1 and TPM1 predicted to enhance actin-tropomyosin interactions.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Type 2 Diabetes-Induced Molecular and Functional Impairment of Adipose Tissue-Derived Mesenchymal Stromal Cells (ASCs) and Interferon Gamma Priming for Enhanced Diabetic ASC-Based Therapy.Stem cell reviews and reports · 2026Article
- NADCells · 2025Review
- Divergent Plasmodium actin residues are essential for filament localization, mosquito salivary gland invasion and malaria transmission.PLoS pathogens · 2022Article
- Structural basis of Naa20 activity towards a canonical NatB substrate.Communications biology · 2021Article
- A functional link between NADThe Journal of biological chemistry · 2018Article
- NatB-mediated protein N-α-terminal acetylation is a potential therapeutic target in hepatocellular carcinoma.Oncotarget · 2017Article
- Mdm20 stimulates polyQ aggregation via inhibiting autophagy through Akt-Ser473 phosphorylation.PloS one · 2013Article
- The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast.Journal of cell science · 2010Article
- The class V myosin motor protein, Myo2, plays a major role in mitochondrial motility in Saccharomyces cerevisiae.The Journal of cell biology · 2008Article
- Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- The novel F-box protein Mfb1p regulates mitochondrial connectivity and exhibits asymmetric localization in yeast.Molecular biology of the cell · 2006Article
- Differential interaction of cardiac, skeletal muscle, and yeast tropomyosins with fluorescent (pyrene235) yeast actin.Biophysical journal · 2006Article
- Physiological importance and identification of novel targets for the N-terminal acetyltransferase NatB.Eukaryotic cell · 2006Article
- X-ray survival characteristics and genetic analysis for nine Saccharomyces deletion mutants that show altered radiation sensitivity.Genetics · 2005Article
- Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway.The Journal of cell biology · 2004Article
- Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Identification of an organelle-specific myosin V receptor.The Journal of cell biology · 2003Article
- Polarized growth and organelle segregation in yeast: the tracks, motors, and receptors.The Journal of cell biology · 2003Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The actin cytoskeleton is required for many aspects of cell division in yeast, including mitochondrial partitioning into growing buds (mitochondrial inheritance). Yeast cells lacking MDM20 function display defects in both mitochondrial inheritance and actin organization, specifically, a lack of visible actin cables and enhanced sensitivity to Latrunculin A. mdm20 mutants also exhibit a temperature-sensitive growth phenotype, which we exploited to isolate second-site suppressor mutations. Nine dominant suppressors selected in an mdm20/mdm20 background rescue temperature-sensitive growth defects and mitochondrial inheritance defects and partially restore actin cables in haploid and diploid mdm20 strains. The suppressor mutations define new alleles of ACT1 and TPM1, which encode actin and the major form of tropomyosin in yeast, respectively. The ACT1 mutations cluster in a region of the actin protein predicted to contact tropomyosin, suggesting that they stabilize actin cables by enhancing actin-tropomyosin interactions. The characteristics of the mutant ACT1 and TPM1 alleles and their potential effects on protein structure and binding are discussed.
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