ArticleJournal of biomolecular NMR2000
The auto-orientation in high magnetic fields of oxidized cytochrome b562 as source of constraints for solution structure determination.
Article in Journal of biomolecular NMR, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 25 citations in OpenAlex.
- NMR-validated structural model for oxidized Rhodopseudomonas palustris cytochrome c(556).Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2004Article
- 15N-1H Residual dipolar coupling analysis of native and alkaline-K79A Saccharomyces cerevisiae cytochrome c.Biophysical journal · 2003Article
- Breaking symmetry in the structure determination of (large) symmetric protein dimers.Journal of biomolecular NMR · 2002Article
- Structure-independent cross-validation between residual dipolar couplings originating from internal and external orienting media.Journal of biomolecular NMR · 2002Article
- Structure refinement of flexible proteins using dipolar couplings: application to the protein p8MTCP1.Journal of biomolecular NMR · 2002Article
- Lanthanide induced residual dipolar couplings for the conformational investigation of peripheral 15NH2 moieties.Journal of biomolecular NMR · 2000Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
15N-1H 1J couplings were measured at 500 MHz and 800 MHz for 15N enriched oxidized cytochrome b562 from E. coli. The magnetic field dependence of 70 1J values, which could be measured without signal overlap, shows that there is a molecular magnetic anisotropy which provides partial molecular orientation in the magnetic field and, consequently, residual dipolar couplings (rdc). The rdc were used as further constraints to improve the existing structure [Arnesano et al. (1999) Biochemistry, 38, 8657-8670] with a protocol which uses the rhombic anisotropy [Banci et al. (1998) J. Am. Ctherz. Soc., 120, 12903-12909]. The overall large molecular magnetic anisotropy has been found to be determined by both the low spin iron (III) and the four helix bundle structure magnetic susceptibility anisotropy contributions.
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