ArticleThe EMBO journal2000
Quorum-sensing signal binding results in dimerization of TraR and its release from membranes into the cytoplasm.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 157 citations in OpenAlex.
- Bioinformatics-Driven, Plant-Based Antibiotic Research Against Quorum Sensing and Biofilm Formation inBiomolecules · 2026Review
- Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria.Molecules (Basel, Switzerland) · 2022Review
- RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids.mBio · 2022Article
- Computer-Aided Rational Engineering of Signal Sensitivity of Quorum Sensing Protein LuxR in a Whole-Cell Biosensor.Frontiers in molecular biosciences · 2021Article
- Characterization of Lactic Acid Bacteria Isolated From the Gastrointestinal Tract of a Wild Boar as Potential Probiotics.Frontiers in veterinary science · 2020Article
- Co-dependent and Interdigitated: Dual Quorum Sensing Systems Regulate Conjugative Transfer of the Ti Plasmid and the At Megaplasmid inFrontiers in microbiology · 2020Article
- Functional characterization of quorum sensing LuxR-type transcriptional regulator, EasR inPeerJ · 2020Article
- An Autoinducer Analogue Reveals an Alternative Mode of Ligand Binding for the LasR Quorum-Sensing Receptor.ACS chemical biology · 2019Article
- Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design.Frontiers in microbiology · 2018Article
- Using synthetic bacterial enhancers to reveal a looping-based mechanism for quenching-like repression.Nature communications · 2016Article
- The repABC Plasmids with Quorum-Regulated Transfer Systems in Members of the Rhizobiales Divide into Two Structurally and Separately Evolving Groups.Genome biology and evolution · 2015Article
- Single cell super-resolution imaging of E. coli OmpR during environmental stress.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Using surface enhanced Raman scattering to analyze the interactions of protein receptors with bacterial quorum sensing modulators.ACS nano · 2015Article
- Identification and characterization of a second quorum-sensing system in Agrobacterium tumefaciens A6.Journal of bacteriology · 2014Article
- An N-acyl homoserine lactone synthase in the ammonia-oxidizing bacterium Nitrosospira multiformis.Applied and environmental microbiology · 2014Article
- Functions and regulation of quorum-sensing in Agrobacterium tumefaciens.Frontiers in plant science · 2014Review
- QsIA disrupts LasR dimerization in antiactivation of bacterial quorum sensing.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Blue light-induced dimerization of a bacterial LOV-HTH DNA-binding protein.Biochemistry · 2013Article
- Prediction by promoter logic in bacterial quorum sensing.PLoS computational biology · 2012Article
- Quorum sensing and expression of virulence in pectobacteria.Sensors (Basel, Switzerland) · 2012Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Promoter binding by TraR and LuxR, the activators of two bacterial quorum-sensing systems, requires their cognate acyl-homoserine lactone (acyl-HSL) signals, but the role the signal plays in activating these transcription factors is not known. Soluble active TraR, when purified from cells grown with the acyl-HSL, contained bound signal and was solely in dimer form. However, genetic and cross-linking studies showed that TraR is almost exclusively in monomer form in cells grown without signal. Adding signal resulted in dimerization of the protein in a concentration-dependent manner. In the absence of signal, monomer TraR localized to the inner membrane while growth with the acyl-HSL resulted in the appearance of dimer TraR in the cytoplasmic compartment. Affinity chromatography indicated that the N-terminus of TraR from cells grown without signal is hidden. Analysis of heterodimers formed between TraR and its deletion mutants localized the dimerization domain to a region between residues 49 and 156. We conclude that binding signal drives dimerization of TraR and its release from membranes into the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.