ArticleApplied and environmental microbiology2000
PCR assays that identify the grapevine dieback fungus Eutypa lata.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 65 citations in OpenAlex.
- Development of SCAR Markers for Genetic Authentication ofJournal of fungi (Basel, Switzerland) · 2024Article
- A Panoramic View on Grapevine Trunk Diseases Threats: Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease.Journal of fungi (Basel, Switzerland) · 2022Review
- The road to molecular identification and detection of fungal grapevine trunk diseases.Frontiers in plant science · 2022Review
- Virtual 2-D map of the fungal proteome.Scientific reports · 2021Article
- Quantitative Assessment of Grapevine Wood Colonization by the Dieback Fungus Eutypa lata.Journal of fungi (Basel, Switzerland) · 2017Article
- Development of specific sequence-characterized amplified region markers for detecting Histoplasma capsulatum in clinical and environmental samples.Journal of clinical microbiology · 2012Article
- Review
- A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata.Journal of experimental botany · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eutypa lata is the causal fungal agent of Eutypa dieback, a serious grapevine necrotic disease. The erratic and delayed (1 to 2 months) appearance of characteristic conidia on culture media and the presence of numerous microorganisms in decaying wood make it difficult either to identify or to detect E. lata in grapevine wood samples. We designed six pairs of PCR primers for diagnosis of E. lata. Three primer pairs were derived from ribosomal DNA internal transcribed spacer sequences, and three pairs were derived from randomly amplified polymorphic DNA fragments. The six primer pairs could be used to amplify DNAs extracted from all of the E. lata isolates tested. They did not amplify DNAs from fungi and bacteria representing more than 50 different species of microorganisms associated with grapevine. We developed a simple protocol, leading to a rapid release of DNA, that enabled us to identify E. lata from pure or mixed cultures as well as from grapevine wood samples. Identification of E. lata in wood was achieved within a few hours, instead of the several weeks required for classical cultures on agar medium. We believe that the procedure described here can be adapted to detect other microorganisms involved in woody plant diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.