ArticleApplied and environmental microbiology2000
Internal trehalose protects endocytosis from inhibition by ethanol in Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 91 citations in OpenAlex.
- Chromosome III Aneuploidy Enhances Ethanol Tolerance in Industrial Saccharomyces cerevisiae by Increasing the TUP1 Copy Number.Microbial biotechnology · 2025Article
- Honey bees are resilient to the long-term presence of alcohol in their diet.Ecotoxicology (London, England) · 2025Article
- Effect of halo-tolerance gene Hal5 on ethanol tolerance ofBBA advances · 2024Article
- Contribution of trehalose to ethanol stress tolerance of Wickerhamomyces anomalus.BMC microbiology · 2023Article
- Wine Yeast Cells Acquire Resistance to Severe Ethanol Stress and Suppress Insoluble Protein Accumulation during Alcoholic Fermentation.Microbiology spectrum · 2022Article
- Mining novelFrontiers in microbiology · 2022Article
- TheMetabolic engineering communications · 2016Article
- Defects in Protein Folding Machinery Affect Cell Wall Integrity and Reduce Ethanol Tolerance in S. cerevisiae.Current microbiology · 2016Article
- Yeast Interactions in Inoculated Wine Fermentation.Frontiers in microbiology · 2016Review
- Sorbitol required for cell growth and ethanol production by Zymomonas mobilis under heat, ethanol, and osmotic stresses.Biotechnology for biofuels · 2013Article
- Exploiting natural variation in Saccharomyces cerevisiae to identify genes for increased ethanol resistance.Genetics · 2010Article
- Heterologous expression of Saccharomyces cerevisiae MPR1 gene confers tolerance to ethanol and L: -azetidine-2-carboxylic acid in Hansenula polymorpha.Journal of industrial microbiology & biotechnology · 2010Article
- Polymorphisms of Saccharomyces cerevisiae genes involved in wine production.Current microbiology · 2009Article
- Genetic dissection of ethanol tolerance in the budding yeast Saccharomyces cerevisiae.Genetics · 2007Article
- Biomass content governs fermentation rate in nitrogen-deficient wine musts.Applied and environmental microbiology · 2004Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endocytosis in Saccharomyces cerevisiae is inhibited by concentrations of ethanol of 2 to 6% (vol/vol), which are lower than concentrations commonly present in its natural habitats. In spite of this inhibition, endocytosis takes place under enological conditions when high concentrations of ethanol are present. Therefore, it seems that yeast has developed some means to circumvent the inhibition. In this work we have investigated this possibility. We identified two stress conditions under which endocytosis was resistant to inhibition by ethanol: fermentation during nitrogen starvation and growth on nonfermentable substrates. Under these conditions, yeast accumulates stress protectors, primarily trehalose and Hsp104, a protein required for yeast to survive ethanol stress. We found the following. (i) The appearance of ethanol resistance was accompanied by trehalose accumulation. (ii) Mutant cells unable to synthesize trehalose also were unable to develop resistance. (iii) Mutant cells that accumulated trehalose during growth on sugars were resistant to ethanol even under this nonstressing condition. (iv) Mutant cells unable to synthesize Hsp104 were able to develop resistance. We conclude that trehalose is the major factor in the protection of endocytosis from ethanol. Our results suggest another important physiological role for trehalose in yeast.
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