ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Biogenesis of regulated exocytotic carriers in neuroendocrine cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 118 citations in OpenAlex.
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- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.The Journal of cell biology · 2022Article
- Coding variants identified in patients with diabetes alter PICK1 BAR domain function in insulin granule biogenesis.The Journal of clinical investigation · 2022Article
- Neurodegenerative VPS41 variants inhibit HOPS function and mTORC1-dependent TFEB/TFE3 regulation.EMBO molecular medicine · 2021Article
- UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes.PLoS genetics · 2017Article
- BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.The Journal of cell biology · 2017Article
- Chlorpromazine Increases the Expression of Polysialic Acid (PolySia) in Human Neuroblastoma Cells and Mouse Prefrontal Cortex.International journal of molecular sciences · 2017Article
- AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.Traffic (Copenhagen, Denmark) · 2014Article
- Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis.PLoS genetics · 2014Article
- BIG3 inhibits insulin granule biogenesis and insulin secretion.EMBO reports · 2014Article
- Self-assembly of VPS41 promotes sorting required for biogenesis of the regulated secretory pathway.Developmental cell · 2013Article
- The functional significance of synaptotagmin diversity in neuroendocrine secretion.Frontiers in endocrinology · 2013Review
- Article
- Widespread dysregulation of peptide hormone release in mice lacking adaptor protein AP-3.PLoS genetics · 2013Article
- Role of adaptor proteins in secretory granule biogenesis and maturation.Frontiers in endocrinology · 2013Article
- RAB-5 and RAB-10 cooperate to regulate neuropeptide release in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cholesterol accumulation increases insulin granule size and impairs membrane trafficking.Traffic (Copenhagen, Denmark) · 2012Article
- Controlling synaptotagmin activity by electrostatic screening.Nature structural & molecular biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Ca(2+)-triggered exocytosis is a hallmark of neurosecretory granules, but the cellular pathway leading to the assembly of these regulated exocytotic carriers is poorly understood. Here we used the pituitary AtT-20 cell line to study the biogenesis of regulated exocytotic carriers involved in peptide hormone secretion. We show that immature secretory granules (ISGs) freshly budded from the trans-Golgi network (TGN) exhibit characteristics of unregulated exocytotic carriers. During a subsequent maturation period they undergo an important switch to become regulated exocytotic carriers. We have identified a novel sorting pathway responsible for this transition. The SNARE proteins, VAMP4 and synaptotagmin IV (Syt IV), enter ISGs initially but are sorted away during maturation. Sorting is achieved by vesicle budding from the ISGs, because it can be inhibited by brefeldin A (BFA). Inhibition of this sorting pathway with BFA arrested the maturing granules in a state that responded poorly to stimuli, suggesting that the transition to regulated exocytotic carriers requires the removal of a putative inhibitor. In support of this, we found that overexpression of Syt IV reduced the stimulus-responsiveness of maturing granules. We conclude that secretory granules undergo a switch from unregulated to regulated secretory carriers during biogenesis. The existence of such a switch may provide a mechanism for cells to modulate their secretory activities under different physiological conditions.
Indexed as
Identifiers
11007891PMC6772794W1485367438What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.