ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
NGF signals through TrkA to increase clathrin at the plasma membrane and enhance clathrin-mediated membrane trafficking.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 133 citations in OpenAlex.
- Two-photon fluorescence imaging and specifically biosensing of norepinephrine on a 100-ms timescale.Nature communications · 2023Article
- Growth cone macropinocytosis of neurotrophin receptor and neuritogenesis are regulated by neuron navigator 1.Molecular biology of the cell · 2022Article
- Activation of neurotrophin signalling with light‑inducible receptor tyrosine kinases.Molecular medicine reports · 2022Article
- Role of adaptin protein complexes in intracellular trafficking and their impact on diseases.Bioengineered · 2021Article
- NGF Signaling in Endosomes.Advances in experimental medicine and biology · 2021Article
- GDNF synthesis, signaling, and retrograde transport in motor neurons.Cell and tissue research · 2020Review
- Article
- Pharmacologically diverse antidepressants facilitate TRKB receptor activation by disrupting its interaction with the endocytic adaptor complex AP-2.The Journal of biological chemistry · 2019Article
- Review
- Genome-wide association study in Finnish twins highlights the connection between nicotine addiction and neurotrophin signaling pathway.Addiction biology · 2019Article
- Ndr2 Kinase Controls Neurite Outgrowth and Dendritic Branching Through αFrontiers in molecular neuroscience · 2018Article
- TrkB neurotrophic activities are blocked by α-synuclein, triggering dopaminergic cell death in Parkinson's disease.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The neurotrophin receptor signaling endosome: Where trafficking meets signaling.Developmental neurobiology · 2017Review
- Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth.Molecular biology of the cell · 2016Article
- Neurotrophin signaling endosomes: biogenesis, regulation, and functions.Current opinion in neurobiology · 2016Review
- IL-7 induces clathrin-mediated endocytosis of CD127 and subsequent degradation by the proteasome in primary human CD8 T cells.Immunology and cell biology · 2016Article
- 7,8-dihydroxyflavone, a small molecular TrkB agonist, is useful for treating various BDNF-implicated human disorders.Translational neurodegeneration · 2016Review
- Biogenesis and function of the NGF/TrkA signaling endosome.International review of cell and molecular biology · 2015Review
- The role of rab proteins in neuronal cells and in the trafficking of neurotrophin receptors.Membranes · 2014Review
- Biochemical and biophysical investigation of the brain-derived neurotrophic factor mimetic 7,8-dihydroxyflavone in the binding and activation of the TrkB receptor.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
Neurotrophin (NT) signals may be moved from axon terminals to neuron cell bodies via signaling endosomes-organelles in which NTs continue to be bound to their activated receptors. Suggesting that clathrin-coated membranes serve as one source of signaling endosomes, in earlier studies we showed that nerve growth factor (NGF) treatment increased clathrin at the plasma membrane and resulted in colocalization of clathrin with TrkA, the receptor tyrosine kinase for NGF. Strikingly, however, we also noted that most clathrin puncta at the surface of NGF-treated cells did not colocalize with TrkA, raising the possibility that NGF induces a general increase in clathrin-coated membrane formation. To explore this possibility further, we examined the distribution of clathrin in NGF- and BDNF-treated cells. NGF signaling in PC12 cells robustly redistributed the adaptor protein AP2 and the clathrin heavy chain (CHC) to surface membranes. Using confocal and epifluorescence microscopy, as well as biochemical assays, we showed the redistribution of clathrin to be attributable to the activation of TrkA. Significantly, NGF signaled through TrkA to induce an increase in clathrin-mediated membrane trafficking, as revealed in the increased endocytosis of transferrin. In that BDNF treatment increased AP2 and clathrin at the surface membranes of hippocampal neurons, these findings may represent a physiologically significant response to NTs. We conclude that NT signaling increases clathrin-coated membrane formation and clathrin-mediated membrane trafficking and speculate that this effect contributes to their trophic actions via the increased internalization of receptors and other proteins that are present in clathrin-coated membranes.
Indexed as
Identifiers
11007890PMC6772792W1577835340What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.