ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Low-affinity blockade of neuronal N-type Ca channels by the spider toxin omega-agatoxin-IVA.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Identification of CaV channel types expressed in muscle afferent neurons.Journal of neurophysiology · 2013Article
- Coactivation of multiple tightly coupled calcium channels triggers spontaneous release of GABA.Nature neuroscience · 2012Article
- Sumatriptan inhibition of N-type calcium channel mediated signaling in dural CGRP terminal fibres.Neuropharmacology · 2012Article
- Peptide neurotoxins that affect voltage-gated calcium channels: a close-up on ω-agatoxins.Toxins · 2011Review
- Ca(2+) and Ca(2+)-activated K(+) channels that support and modulate transmitter release at the olivocochlear efferent-inner hair cell synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Acute restraint stress enhances calcium mobilization and glutamate exocytosis in cerebrocortical synaptosomes from mice.Neurochemical research · 2010Article
- Regulation of glutamatergic and GABAergic neurotransmission in the chick nucleus laminaris: role of N-type calcium channels.Neuroscience · 2009Article
- Maturation of rat cerebellar Purkinje cells reveals an atypical Ca2+ channel current that is inhibited by omega-agatoxin IVA and the dihydropyridine (-)-(S)-Bay K8644.The Journal of physiology · 2007Article
- Silent plateau potentials, rhythmic bursts, and pacemaker firing: three patterns of activity that coexist in quadristable subthalamic neurons.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Molecular pharmacology of high voltage-activated calcium channels.Journal of bioenergetics and biomembranes · 2003Review
- Melanin-concentrating hormone depresses L-, N-, and P/Q-type voltage-dependent calcium channels in rat lateral hypothalamic neurons.The Journal of physiology · 2002Article
- Interactions among toxins that inhibit N-type and P-type calcium channels.The Journal of general physiology · 2002Article
- Interaction of SNX482 with domains III and IV inhibits activation gating of alpha(1E) (Ca(V)2.3) calcium channels.Biophysical journal · 2001Article
- Activation of the cannabinoid receptor type 1 decreases glutamatergic and GABAergic synaptic transmission in the lateral amygdala of the mouse.Learning & memory (Cold Spring Harbor, N.Y.)Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The recognition of neuronal Ca channel diversity has led to considerable efforts to identify useful classification criteria. Here, we revisit the pharmacological definition of P- and Q-type Ca channels, which is based on their respective high and low sensitivity to the spider omega-agatoxin-IVA (omega-Aga-IVA), using whole-cell recordings of the Ca channel currents carried by 5 mM Ba(2+) in isolated rat subthalamic and sympathetic neurons. In subthalamic neurons, omega-Aga-IVA (1 microM) targeted multiple Ca channels. One population was blocked with high potency. These channels carried 50.4 +/- 3.4% (n = 5) of the control current and showed the same inactivation kinetics and voltage-dependent high affinity for omega-Aga-IVA as do prototypic P-type Ca channels. Other Ca channels were targeted with weaker potency. This heterogeneous population contributed to 14.0 +/- 1.7% (n = 5) of the control current. It included N-type Ca channels as well as high-threshold Ca channels that displayed the pharmacological signature of Q-type Ca channels but resembled P-type Ca channels in their gating properties. N-type Ca current block by omega-Aga-IVA (1 microM) was further investigated in sympathetic neurons, which mainly express this Ca channel type. Block was incomplete ( approximately 30% of the control current). Its relief at positive potentials was consistent with omega-Aga-IVA acting as a channel-gating modifier. These effects did not reflect a complete loss of selectivity, because omega-Aga-IVA (1 microM) had no effect on subthalamic Na and K currents or their T- and L-type Ca currents. Our data confirm that omega-Aga-IVA is a selective P-type Ca channel blocker. However, its diminished selectivity in the micromolar range limits its usefulness for functional studies of Q-type Ca channels.
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Identifiers
11007873PMC6772783W1925093345What OpenQuestion holds
Registered trials
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