ArticleThe Plant cell2000
Biogenesis of the chloroplast-encoded D1 protein: regulation of translation elongation, insertion, and assembly into photosystem II.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 168 citations in OpenAlex.
- From proton microdomains to protein migration: hypothesis for ΔpH-controlled PSII relocation.Photosynthesis research · 2026Article
- Light intensity influences carotenoid accumulation and modulates the expression of photosynthetic genes in Euglena sanguinea.Photosynthesis research · 2025Article
- Defining the heterogeneous composition of Arabidopsis thylakoid membrane.The Plant journal : for cell and molecular biology · 2025Article
- Temperature-driven changes in membrane fluidity differentially impact FILAMENTATION TEMPERATURE-SENSITIVE H2-mediated photosystem II repair.The Plant cell · 2024Article
- Experimental approaches to studying translation in plant semi-autonomous organelles.Journal of experimental botany · 2024Review
- Photosynthesis: Genetic Strategies Adopted to Gain Higher Efficiency.International journal of molecular sciences · 2024Review
- Thylakoid protein FPB1 synergistically cooperates with PAM68 to promote CP47 biogenesis and Photosystem II assembly.Nature communications · 2024Article
- The best of both worlds: photosynthesis and Solanaceae biodiversity seeking a sustainable food and cosmetic industry.Frontiers in plant science · 2024Article
- The sensitivity of photosynthesis to magnesium deficiency differs between rice (Frontiers in plant science · 2023Article
- Cotranslational Mechanisms of Protein Biogenesis and Complex Assembly in Eukaryotes.Annual review of biomedical data science · 2022Review
- How Light Reactions of Photosynthesis in C4 Plants Are Optimized and Protected under High Light Conditions.International journal of molecular sciences · 2022Review
- Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex inFrontiers in plant science · 2022Article
- Light-inducedProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Limited Responsiveness of Chloroplast Gene Expression during Acclimation to High Light in Tobacco.Plant physiology · 2020Article
- OHP1, OHP2, and HCF244 Form a Transient Functional Complex with the Photosystem II Reaction Center.Plant physiology · 2019Article
- Multilevel effects of light on ribosome dynamics in chloroplasts program genome-wide and psbA-specific changes in translation.PLoS genetics · 2018Article
- TEF30 Interacts with Photosystem II Monomers and Is Involved in the Repair of Photodamaged Photosystem II in Chlamydomonas reinhardtii.Plant physiology · 2016Article
- Photoactivation: The Light-Driven Assembly of the Water Oxidation Complex of Photosystem II.Frontiers in plant science · 2016Review
- Photoprotection as a Trait for Rice Yield Improvement: Status and Prospects.Rice (New York, N.Y.) · 2015Article
- Ribosome nascent chain complexes of the chloroplast-encoded cytochrome b6 thylakoid membrane protein interact with cpSRP54 but not with cpSecY.Journal of bioenergetics and biomembranes · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of translation elongation, membrane insertion, and assembly of the chloroplast-encoded D1 protein of photosystem II (PSII) was studied using a chloroplast translation system in organello. Translation elongation of D1 protein was found to be regulated by (1) a redox component that can be activated not only by photosynthetic electron transfer but also by reduction with DTT; (2) the trans-thylakoid proton gradient, which is absolutely required for elongation of D1 nascent chains on the thylakoid membrane; and (3) the thiol reactants N-ethylmaleimide (NEM) and iodosobenzoic acid (IBZ), which inhibit translation elongation with concomitant accumulation of distinct D1 pausing intermediates. These results demonstrate that D1 translation elongation and membrane insertion are tightly coupled and highly regulated processes in that proper insertion is a prerequisite for translation elongation of D1. Cotranslational and post-translational assembly steps of D1 into PSII reaction center and core complexes occurred independently of photosynthetic electron transfer or trans-thylakoid proton gradient but were strongly affected by the thiol reactants DTT, NEM, and IBZ. These compounds reduced the stability of the early PSII assembly intermediates, hampered the C-terminal processing of the precursor of D1, and prevented the post-translational reassociation of CP43, indicating a strong dependence of the D1 assembly steps on proper redox conditions and the formation of disulfide bonds.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.