ArticleThe Plant cell2000
Identification and characterization of a novel microtubule-based motor associated with membranous organelles in tobacco pollen tubes.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 68 citations in OpenAlex.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Microtubule-associated protein PlWDL2 positively promotes stem strength in herbaceous peony.Planta · 2025Article
- From the archives: Roles of microtubule-associated proteins in organelle movement, tip growth, and phragmoplast architecture.The Plant cell · 2024Article
- Unraveling the interplay of kinesin-1, tau, and microtubules in neurodegeneration associated with Alzheimer's disease.Frontiers in cellular neuroscience · 2024Review
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- Biochemical and cytological interactions between callose synthase and microtubules in the tobacco pollen tube.Plant cell reports · 2022Article
- The legacy of kinesins in the pollen tube 30 years later.Cytoskeleton (Hoboken, N.J.) · 2022Review
- Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes.Open biology · 2018Article
- Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.Annals of botany · 2018Article
- Spermine Regulates Pollen Tube Growth by Modulating CaFrontiers in plant science · 2017Article
- Heat stress affects the cytoskeleton and the delivery of sucrose synthase in tobacco pollen tubes.Planta · 2016Article
- Emerging roles for microtubules in angiosperm pollen tube growth highlight new research cues.Frontiers in plant science · 2015Article
- Sucrose concentration in the growth medium affects the cell wall composition of tobacco pollen tubes.Plant reproduction · 2014Article
- The Arabidopsis kinesin gene AtKin-1 plays a role in the nuclear division process during megagametogenesis.Plant cell reports · 2014Article
- Are kinesins required for organelle trafficking in plant cells?Frontiers in plant science · 2012Article
- Microtubule motors and pollen tube growth--still an open question.Protoplasma · 2010Review
- Overexpression of PwTUA1, a pollen-specific tubulin gene, increases pollen tube elongation by altering the distribution of alpha-tubulin and promoting vesicle transport.Journal of experimental botany · 2009Article
- Costal2 functions as a kinesin-like protein in the hedgehog signal transduction pathway.Current biology : CB · 2008Article
- Differential expansion and expression of alpha- and beta-tubulin gene families in Populus.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen tube growth depends on the differential distribution of organelles and vesicles along the tube. The role of microtubules in organelle movement is uncertain, mainly because information at the molecular level is limited. In an effort to understand the molecular basis of microtubule-based movement, we isolated from tobacco pollen tubes polypeptides that cosediment with microtubules in an ATP-dependent manner. Major polypeptides released from microtubules by ATP (ATP-MAPs) had molecular masses of 90, 80, and 41 kD. Several findings indicate that the 90-kD ATP-MAP is a kinesin-related motor: binding of the polypeptide to microtubules was enhanced by the nonhydrolyzable ATP analog AMP-PNP; the 90-kD polypeptide reacted specifically with a peptide antibody directed against a highly conserved region in the motor domain of the kinesin superfamily; purified 90-kD ATP-MAP induced microtubules to glide in motility assays in vitro; and the 90-kD ATP-MAP cofractionated with microtubule-activated ATPase activity. Immunolocalization studies indicated that the 90-kD ATP-MAP binds to organelles associated with microtubules in the cortical region of the pollen tube. These findings suggest that the 90-kD ATP-MAP is a kinesin-related microtubule motor that moves organelles in the cortex of growing pollen tubes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.