ArticleInvestigative ophthalmology & visual science2000
Erk and PI-3 kinase are necessary for collagen binding and actin reorganization in corneal epithelia.
Article in Investigative ophthalmology & visual science, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Effect of the Expression ofFrontiers in genetics · 2021Article
- Stimulation of transforming growth factor-beta-1 and contact with type I collagen cooperatively facilitate irreversible transdifferentiation in proximal tubular cells.Biomedical journal · 2016Article
- Article
- h3/Acidic calponin: an actin-binding protein that controls extracellular signal-regulated kinase 1/2 activity in nonmuscle cells.Molecular biology of the cell · 2010Article
- Prox1 Facilitates Transfected CHO Cell Proliferation through Activation of the AKT Signaling Pathway.International journal of biomedical science : IJBS · 2010Article
- Embryonic chick corneal epithelium: a model system for exploring cell-matrix interactions.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Review
- Nicotine inhibits human gingival fibroblast migration via modulation of Rac signalling pathways.Journal of clinical periodontology · 2005Article
- ROCK inhibitor (Y27632) increases apoptosis and disrupts the actin cortical mat in embryonic avian corneal epithelium.Developmental dynamics : an official publication of the American Association of Anatomists · 2004Article
- Extracellular signal-regulated protein kinase activation during reoxygenation is required to restore ischaemia-induced endothelial barrier failure.The Biochemical journal · 2002Article
- ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent.Investigative ophthalmology & visual science · 2002Article
- Approaches to studying cellular signaling: a primer for morphologists.The Anatomical record · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeIt was recently shown that phosphatidylinositol-(PI)3 kinase is upregulated in wounded rabbit corneal epithelia. Extracellular signal-regulated kinase (erk)-1 and -2 proteins and PI-3 kinase were activated in embryonic corneal epithelia after 1-hour stimulation by type I collagen. In the current investigation specific inhibitors of PI-3 kinase and mitogen-activated kinase-kinase (MEK-1 kinase) were used to determine the role of these signaling molecules in actin reorganization and collagen binding to isolated sheets of corneal epithelial tissue.
methodsEffects of specific PI-3 kinase and MEK-1 inhibitors (LY294002, PD98059, respectively) were investigated in embryonic corneal epithelial tissues. Avian embryonic corneal epithelia were isolated as tissue sheets, organ cultured in the presence of these specific inhibitors, and stimulated with type I collagen. The tissues were evaluated for collagen-stimulated actin reorganization, erk-1 and -2 and PI-3 kinase activity, total filamentous actin accumulation, and collagen binding.
resultsThe MEK-1 inhibitor PD98059 decreased erk-1 and -2 phosphorylation and blocked actin reorganization in a dose-dependent manner. The PI-3 kinase 85-kDa subunit was decreased 25% in LY294002-treated tissue, and collagen binding also decreased significantly in tissues treated with MEK-1 and PI-3 kinase inhibitors compared with control tissues. In addition, both inhibitors blocked actin cortical mat reorganization. CONCLUSIONS; PI-3 kinase and erk-1 and -2 signaling pathways are activated and necessary for collagen binding and integrin-mediated actin reorganization in embryonic avian corneal epithelium.
Indexed as
Identifiers
11006227PMC2864550W2168589324What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.