ArticleProceedings of the National Academy of Sciences of the United States of America2000
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 159 citations in OpenAlex.
- Host serum-circulating flavivirus NS1 co-opts mosquito miRNA processing to suppress early immune defense.Nature communications · 2026Article
- Arthropod promoters for genetic control of disease vectors.Trends in parasitology · 2024Review
- An 11-point time course midgut transcriptome across 72 h after bloodfeeding provides detailed temporal resolution of transcript expression in the arbovirus vector,Genome research · 2023Article
- Expression of anti-chikungunya single-domain antibodies in transgenicFrontiers in microbiology · 2023Article
- Use of Insect Promoters in Genetic Engineering to Control Mosquito-Borne Diseases.Biomolecules · 2022Review
- Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes.Scientific reports · 2022Article
- The Aedes aegypti siRNA pathway mediates broad-spectrum defense against human pathogenic viruses and modulates antibacterial and antifungal defenses.PLoS biology · 2022Article
- New weapons to fight malaria transmission: A historical view.Entomological research · 2022Review
- Structure ofLife science alliance · 2022Article
- The impact of transgenesis on mosquito fitness: A review.Frontiers in insect science · 2022Review
- Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus.Scientific reports · 2021Article
- Structure of Aedes aegypti carboxypeptidase B1-inhibitor complex uncover the disparity between mosquito and non-mosquito insect carboxypeptidase inhibition mechanism.Protein science : a publication of the Protein Society · 2021Article
- Identification of new Anopheles gambiae transcriptional enhancers using a cross-species prediction approach.Insect molecular biology · 2021Article
- The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in TransgenicViruses · 2020Article
- The Developmental Transcriptome ofG3 (Bethesda, Md.) · 2020Article
- Broad dengue neutralization in mosquitoes expressing an engineered antibody.PLoS pathogens · 2020Article
- Engineered action at a distance: Blood-meal-inducible paralysis in Aedes aegypti.PLoS neglected tropical diseases · 2019Article
- Engineered resistance to Zika virus in transgenicProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Functional analysis of the promoter of an early zygotic gene KLC2 in Aedes aegypti.Parasites & vectors · 2018Article
- Field assessment of potential sugar feeding stations for disseminating bacteria in a paratransgenic approach to control malaria.Malaria journal · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on the characterization in transgenic Aedes aegypti of two mosquito gut-specific promoters. A 1.4-kb DNA fragment adjacent to the 5' end of the coding region of the Ae. aegypti carboxypeptidase (AeCP) gene and a corresponding 3.4-kb DNA fragment at the 5' end of the Anopheles gambiae carboxypeptidase (AgCP) gene were linked to a firefly luciferase reporter gene and introduced into the Ae. aegypti germ line by using Hermes and mariner (Mos1) transposons. Six independent transgenic lines were obtained with the AeCP construct and one with the AgCP construct. Luciferase mRNA and protein were abundantly expressed in the guts of transgenic mosquitoes in four of the six AeCP lines and in the AgCP line. Expression of the reporter gene was gut-specific and reached peak levels at about 24 h post-blood ingestion. The AeCP and AgCP promoters can be used to drive the expression of genes that hinder parasite development in the mosquito gut.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.