ArticleCell stress & chaperones2000
Potential targets for HSF1 within the preinitiation complex.
Article in Cell stress & chaperones, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Functional implications of paralog genes in polyglutamine spinocerebellar ataxias.Human genetics · 2023Review
- AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation.The FEBS journal · 2022Article
- Bromodomain factors of BET family are new essential actors of pericentric heterochromatin transcriptional activation in response to heat shock.Scientific reports · 2017Article
- E2F coregulates an essential HSF developmental program that is distinct from the heat-shock response.Genes & development · 2016Article
- Article
- Nucleosome distortion as a possible mechanism of transcription activation domain function.Epigenetics & chromatin · 2016Review
- Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation.Molecular and cellular biology · 2015Article
- Polymorphisms in human heat shock factor-1 and analysis of potential biological consequences.Cell stress & chaperones · 2015Article
- Disease-linked microRNA-21 exhibits drastically reduced mRNA binding and silencing activity in healthy mouse liver.RNA (New York, N.Y.) · 2012Article
- Inducible hsp70 in the regulation of cancer cell survival: analysis of chaperone induction, expression and activity.Cancers · 2011Article
- Prostaglandin E2 potentiates heat shock-induced heat shock protein 72 expression in A549 cells.Prostaglandins & other lipid mediators · 2010Article
- Febrile range temperature represses TNF-alpha gene expression in LPS-stimulated macrophages by selectively blocking recruitment of Sp1 to the TNF-alpha promoter.Cell stress & chaperones · 2010Article
- Heat shock factors: integrators of cell stress, development and lifespan.Nature reviews. Molecular cell biology · 2010Review
- Febrile-range temperature modifies cytokine gene expression in LPS-stimulated macrophages by differentially modifying NF-{kappa}B recruitment to cytokine gene promoters.American journal of physiology. Cell physiology · 2010Article
- Transcriptional malfunctioning of heat shock protein gene expression in spinocerebellar ataxias.Cerebellum (London, England) · 2007Review
- Could heat shock transcription factors function as hydrogen peroxide sensors in plants?Annals of botany · 2006Review
- Functional interaction between two transcription factors involved in the developmental regulation of a small heat stress protein gene promoter.Plant physiology · 2005Article
- Plant class B HSFs inhibit transcription and exhibit affinity for TFIIB and TBP.Plant molecular biology · 2004Article
- Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?Cell stress & chaperones · 2001Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-protein interactions between human heat shock transcription factor 1 (hHSF1) and general transcription factors TFIIA-gamma, TFIIB, TBP, TAF(II)32, and TAF(II)55 and positive coactivator PC4 were characterized in order to identify potential targets of contact in the transcriptional preinitiation complex. These contacts represent one of the final steps in the signal transfer of heat stress to the transcriptional apparatus. TATA-binding protein (TBP) and transcription factor IIB (TFIIB) were identified as major targets for HSF1 transcriptional activation domains AD1 and AD2 based on in vitro interaction assays. TBP showed affinity for AD2 and a fragment containing AD1, while the core domain of TFIIB interacted primarily with the AD1 fragment. Interactions were also detected between full-length HSF1 and the small subunit (gamma) of TFIIA. PC4 interacted weakly with HSF2 and showed even less affinity for HSF1. Coimmunoprecipitation of transiently expressed TBP in HeLa cells demonstrated that HSF1 AD2 and AD1+AD2 are able to bind TBP in vivo. Assays based on transcriptional interference confirmed predictions that both TBP and TFIIB can interact with HSF1 activation domains in HeLa cells. The negative regulatory region (NR) of HSF1 did not interact with any general factors tested in vitro but did bind TFIID in nuclear extracts through contacts that probably involve TATA associated proteins (TAFs). These results suggest a model for transcriptional regulation by HSF1 that involves a shift between formation of dysfunctional TFIID complexes with the NR and transcriptionally competent complexes with the C-terminal activation domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.