ArticleJournal of bacteriology2000
Role of the Escherichia coli nucleotide excision repair proteins in DNA replication.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 76 citations in OpenAlex.
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- Cellular and Molecular Mechanisms of Oxidative DNA Damage and Repair.Medicina (Kaunas, Lithuania) · 2025Review
- Interrogating the substrate specificity landscape of UvrC reveals novel insights into its non-canonical function.Biophysical journal · 2022Article
- The Effects of Freeze-Thaw and UVC Radiation on Microbial Survivability in a Selected Mars-like Environment.Microorganisms · 2022Article
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- Transition of Serotype 35B Pneumococci From Commensal to Prevalent Virulent Strain in Children.Frontiers in cellular and infection microbiology · 2021Article
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- Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity.Nucleic acids research · 2015Article
- Active displacement of RecA filaments by UvrD translocase activity.Nucleic acids research · 2015Article
- Genetically engineered synthetic miniaturized versions of Plasmodium falciparum UvrD helicase are catalytically active.PloS one · 2014Article
- Integrated stress response of Escherichia coli to methylglyoxal: transcriptional readthrough from the nemRA operon enhances protection through increased expression of glyoxalase I.Molecular microbiology · 2013Article
- UvrD2 is essential in Mycobacterium tuberculosis, but its helicase activity is not required.Journal of bacteriology · 2011Article
- Modulation of UvrD helicase activity by covalent DNA-protein cross-links.The Journal of biological chemistry · 2010Article
- Article
- Stimulation of UvrD helicase by UvrAB.The Journal of biological chemistry · 2009Article
- Particulate matter inhibits DNA repair and enhances mutagenesis.Mutation research · 2008Article
- Cooperative damage recognition by UvrA and UvrB: identification of UvrA residues that mediate DNA binding.DNA repair · 2008Article
- Crystal structure of Bacillus stearothermophilus UvrA provides insight into ATP-modulated dimerization, UvrB interaction, and DNA binding.Molecular cell · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA polymerase I (PolI) functions both in nucleotide excision repair (NER) and in the processing of Okazaki fragments that are generated on the lagging strand during DNA replication. Escherichia coli cells completely lacking the PolI enzyme are viable as long as they are grown on minimal medium. Here we show that viability is fully dependent on the presence of functional UvrA, UvrB, and UvrD (helicase II) proteins but does not require UvrC. In contrast, delta polA cells grow even better when the uvrC gene has been deleted. Apparently UvrA, UvrB, and UvrD are needed in a replication backup system that replaces the PolI function, and UvrC interferes with this alternative replication pathway. With specific mutants of UvrC we could show that the inhibitory effect of this protein is related to its catalytic activity that on damaged DNA is responsible for the 3' incision reaction. Specific mutants of UvrA and UvrB were also studied for their capacity to support the PolI-independent replication. Deletion of the UvrC-binding domain of UvrB resulted in a phenotype similar to that caused by deletion of the uvrC gene, showing that the inhibitory incision activity of UvrC is mediated via binding to UvrB. A mutation in the N-terminal zinc finger domain of UvrA does not affect NER in vivo or in vitro. The same mutation, however, does give inviability in combination with the delta polA mutation. Apparently the N-terminal zinc-binding domain of UvrA has specifically evolved for a function outside DNA repair. A model for the function of the UvrA, UvrB, and UvrD proteins in the alternative replication pathway is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.