ArticleJournal of bacteriology2000
Isolation of regulated genes of the cyanobacterium Synechocystis sp. strain PCC 6803 by differential display.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Spirulina/Arthrospira/Limnospira-Three Names of the Single Organism.Foods (Basel, Switzerland) · 2024Review
- Fourier transform infrared spectroscopy and machine learning for Porphyromonas gingivalis detection in oral bacteria.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Regulation of the carbon-concentrating mechanism in the cyanobacterium Synechocystis sp. PCC6803 in response to changing light intensity and inorganic carbon availability.Photosynthesis research · 2013Article
- The quantitative proteomic response of Synechocystis sp. PCC6803 to phosphate acclimation.Aquatic biosystems · 2013Article
- Microevolution in cyanobacteria: re-sequencing a motile substrain of Synechocystis sp. PCC 6803.DNA research : an international journal for rapid publication of reports on genes and genomes · 2012Article
- The antimicrobial profile of extracts of a Phormidium-like cyanobacterium changes with phosphate levels.World journal of microbiology & biotechnology · 2012Article
- Effect of nitrate availability on expression of multi sensor histidine kinase gene in Spirulina platensis PCC 7345.Physiology and molecular biology of plants : an international journal of functional plant biology · 2011Article
- Functional characterization of Synechocystis sp. strain PCC 6803 pst1 and pst2 gene clusters reveals a novel strategy for phosphate uptake in a freshwater cyanobacterium.Journal of bacteriology · 2010Article
- Ecological genomics of marine picocyanobacteria.Microbiology and molecular biology reviews : MMBR · 2009Review
- In situ analysis of nitrogen fixation and metabolic switching in unicellular thermophilic cyanobacteria inhabiting hot spring microbial mats.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Nitrate and phosphate affect cultivability of cyanobacteria from environments with low nutrient levels.Applied and environmental microbiology · 2005Article
- Identification of an Na(+)-dependent transporter associated with saxitoxin-producing strains of the cyanobacterium Anabaena circinalis.Applied and environmental microbiology · 2004Article
- Identification of differential gene expression in bacteria associated with coral black band disease by using RNA-arbitrarily primed PCR.Applied and environmental microbiology · 2004Article
- Consequences of a deletion in dspA on transcript accumulation in Synechocystis sp. strain PCC6803.Journal of bacteriology · 2004Article
- Analysis of light and CO(2) regulation in Chlamydomonas reinhardtii using genome-wide approaches.Photosynthesis research · 2003Article
- mRNA differential display in a microbial enrichment culture: simultaneous identification of three cyclohexanone monooxygenases from three species.Applied and environmental microbiology · 2003Article
- Genome-wide dynamic transcriptional profiling of the light-to-dark transition in Synechocystis sp. strain PCC 6803.Journal of bacteriology · 2002Article
- Evolution of genome-phenome diversity under environmental stress.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- Highly expressed and alien genes of the Synechocystis genome.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Global identification of differentially regulated genes in prokaryotes is constrained because the mRNA does not have a 3' polyadenylation extension; this precludes specific separation of mRNA from rRNA and tRNA and synthesis of cDNAs from the entire mRNA population. Knowledge of the entire genome sequence of Synechocystis sp. strain PCC 6803 has enabled us to develop a differential display procedure that takes advantage of a short palindromic sequence that is dispersed throughout the Synechocystis sp. strain PCC 6803 genome. This sequence, designated the HIP (highly iterated palindrome) element, occurs in approximately half of the Synechocystis sp. strain PCC 6803 genes but is absent in rRNA and tRNA genes. To determine the feasibility of exploiting the HIP element, alone or in combination with specific primer subsets, for analyzing differential gene expression, we used HIP-based primers to identify light intensity-regulated genes. Several gene fragments, including those encoding ribosomal proteins and phycobiliprotein subunits, were differentially amplified from RNA templates derived from cells grown in low light or exposed to high light for 3 h. One novel finding was that expression of certain genes of the pho regulon, which are under the control of environmental phosphate levels, were markedly elevated in high light. High-light activation of pho regulon genes correlated with elevated growth rates that occur when the cells are transferred from low to high light. These results suggest that in high light, the rate of growth of Synechocystis sp. strain PCC 6803 exceeds its capacity to assimilate phosphate, which, in turn, may trigger a phosphate starvation response and activation of the pho regulon.
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