ArticleJournal of bacteriology2000
Synergistic hydrolysis of carboxymethyl cellulose and acid-swollen cellulose by two endoglucanases (CelZ and CelY) from Erwinia chrysanthemi.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 92 citations in OpenAlex.
- EngineeringFrontiers in bioengineering and biotechnology · 2026Article
- A concise review on waste biomass valorization through thermochemical conversion.Current research in microbial sciences · 2024Review
- Pretreatment of Lignocellulosic Materials as Substrates for Fermentation Processes.Molecules (Basel, Switzerland) · 2018Review
- Structure and function of a novel GH8 endoglucanase from the bacterial cellulose synthase complex of Raoultella ornithinolytica.PloS one · 2017Article
- Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed inBiotechnology for biofuels · 2017Article
- Periplasmic Cytophaga hutchinsonii Endoglucanases Are Required for Use of Crystalline Cellulose as the Sole Source of Carbon and Energy.Applied and environmental microbiology · 2016Article
- Microbial diversity in various types of paper mill sludge: identification of enzyme activities with potential industrial applications.SpringerPlus · 2016Article
- Cellulose degradation: a therapeutic strategy in the improved treatment of Acanthamoeba infections.Parasites & vectors · 2015Review
- Characterization of cellulolytic activities of environmental bacterial consortia from an Argentinian native forest.Current microbiology · 2013Article
- Characteristics of a β-1,4-D endoglucanase from Trichoderma virens wholly applied in a palm-fruit husk-based diet for poultry layers.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- A cellular automaton model of crystalline cellulose hydrolysis by cellulases.Biotechnology for biofuels · 2011Article
- Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars.Plant biotechnology journal · 2010Article
- Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence.Genetics and molecular biology · 2010Article
- Fungal biodegradation and enzymatic modification of lignin.International journal of biochemistry and molecular biology · 2010Article
- Fungal bioconversion of lignocellulosic residues; opportunities & perspectives.International journal of biological sciences · 2009Review
- Synthetic biology and biomass conversion: a match made in heaven?Journal of the Royal Society, Interface · 2009Review
- Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives.Journal of industrial microbiology & biotechnology · 2008Review
- Microbial cellulose utilization: fundamentals and biotechnology.Microbiology and molecular biology reviews : MMBR · 2002Review
- Gene integration and expression and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2.Applied and environmental microbiology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Erwinia chrysanthemi produces a battery of hydrolases and lyases which are very effective in the maceration of plant cell walls. Although two endoglucanases (CelZ and CelY; formerly EGZ and EGY) are produced, CelZ represents approximately 95% of the total carboxymethyl cellulase activity. In this study, we have examined the effectiveness of CelY and CelZ alone and of combinations of both enzymes using carboxymethyl cellulose (CMC) and amorphous cellulose (acid-swollen cellulose) as substrates. Synergy was observed with both substrates. Maximal synergy (1.8-fold) was observed for combinations containing primarily CelZ; the ratio of enzyme activities produced was similar to those produced by cultures of E. chrysanthemi. CelY and CelZ were quite different in substrate preference. CelY was unable to hydrolyze soluble cellooligosaccharides (cellotetraose and cellopentaose) but hydrolyzed CMC to fragments averaging 10.7 glucosyl units. In contrast, CelZ readily hydrolyzed cellotetraose, cellopentaose, and amorphous cellulose to produce cellobiose and cellotriose as dominant products. CelZ hydrolyzed CMC to fragments averaging 3.6 glucosyl units. In combination, CelZ and CelY hydrolyzed CMC to products averaging 2.3 glucosyl units. Synergy did not require the simultaneous presence of both enzymes. Enzymatic modification of the substrate by CelY increased the rate and extent of hydrolysis by CelZ. Full synergy was retained by the sequential hydrolysis of CMC, provided CelY was used as the first enzyme. A general mechanism is proposed to explain the synergy between these two enzymes based primarily on differences in substrate preference.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.