ArticleMolecular and cellular biology2000
Reprogrammable recognition codes in bicoid homeodomain-DNA interaction.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Phylogenetic and mutational analyses of human LEUTX, a homeobox gene implicated in embryogenesis.Scientific reports · 2018Article
- Ancient mechanisms for the evolution of the bicoid homeodomain's function in fly development.eLife · 2018Article
- Probing the impact of temperature on molecular events in a developmental system.Scientific reports · 2015Article
- Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2.Experimental cell research · 2015Article
- Identification of a novel frameshift mutation in PITX2 gene in a Chinese family with Axenfeld-Rieger syndrome.Journal of Zhejiang University. Science. B · 2014Article
- Structural and biophysical insights into the ligand-free Pitx2 homeodomain and a ring dermoid of the cornea inducing homeodomain mutant.Biochemistry · 2012Article
- Article
- Paired-like homeodomain transcription factors 1 and 2 regulate follicle-stimulating hormone beta-subunit transcription through a conserved cis-element.Endocrinology · 2008Article
- A novel PITX2 mutation in a Chinese family with Axenfeld-Rieger syndrome.Molecular vision · 2008Article
- OTX5 regulates pineal expression of the zebrafish REV-ERB alpha through a new DNA binding site.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Enhancer sequences influence the role of the amino-terminal domain of bicoid in transcription.Molecular and cellular biology · 2003Article
- Dlx proteins position the neural plate border and determine adjacent cell fates.Development (Cambridge, England) · 2003Article
- Pitx2a expression alters actin-myosin cytoskeleton and migration of HeLa cells through Rho GTPase signaling.Molecular biology of the cell · 2002Article
- A single Hox3 gene with composite bicoid and zerknullt expression characteristics in non-Cyclorrhaphan flies.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Combinatorial interactions of two amino acids with a single base pair define target site specificity in plant dimeric homeodomain proteins.Nucleic acids research · 2001Article
- PITX2 regulates procollagen lysyl hydroxylase (PLOD) gene expression: implications for the pathology of Rieger syndrome.The Journal of cell biology · 2001Article
- Target selectivity of bicoid is dependent on nonconsensus site recognition and protein-protein interaction.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We describe experiments to determine how the homeodomain of the Drosophila morphogenetic protein Bicoid recognizes different types of DNA sequences found in natural enhancers. Our chemical footprint analyses reveal that the Bicoid homeodomain makes both shared and distinct contacts with a consensus site A1 (TAATCC) and a nonconsensus site X1 (TAAGCT). In particular, the guanine of X1 at position 4 (TAAGCT) is protected by Bicoid homeodomain. We provide further evidence suggesting that the unique arginine at position 54 (Arg 54) of the Bicoid homeodomain enables the protein to recognize X1 by specifically interacting with this position 4 guanine. We also describe experiments to analyze the contribution of artificially introduced Arg 54 to DNA recognition by other Bicoid-related homeodomains, including that from the human disease protein Pitx2. Our experiments demonstrate that the role of Arg 54 varies depending on the exact homeodomain framework and DNA sequences. Together, our results suggest that Bicoid and its related homeodomains utilize distinct recognition codes to interact with different DNA sequences, underscoring the need to study DNA recognition by Bicoid-class homeodomains in an individualized manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.