ArticleNucleic acids research2000
CArG binding factor A (CBF-A) is involved in transcriptional regulation of the rat Ha-ras promoter.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 36 citations in OpenAlex.
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- Novel TCF4:TCF12 heterodimer inhibits glioblastoma growth.Molecular oncology · 2024Article
- TWIST1 Heterodimerization with E12 Requires Coordinated Protein Phosphorylation to Regulate Periostin Expression.Cancers · 2019Article
- Hnrnpab regulates neural cell motility through transcription of Eps8.RNA (New York, N.Y.) · 2019Article
- Increased age of transformed mouse neural progenitor/stem cells recapitulates age-dependent clinical features of human glioma malignancy.Aging cell · 2012Article
- Hnrpab regulates neural development and neuron cell survival after glutamate stimulation.RNA (New York, N.Y.) · 2012Article
- Characterization of nuclear factors modulating the apolipoprotein D promoter during growth arrest: implication of PARP-1, APEX-1 and ERK1/2 catalytic activities.Biochimica et biophysica acta · 2010Article
- In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences.Molecular biology of the cell · 2008Article
- A proximal activator of transcription in epithelial-mesenchymal transition.The Journal of clinical investigation · 2007Article
- Transcriptional regulation of epithelial-mesenchymal transition.The Journal of clinical investigation · 2007Article
- Transcriptional analysis of avian embryonic tissues following infection with avian infectious bronchitis virus.Virus research · 2005Article
- Impaired repression at a vasopressin promoter polymorphism underlies overexpression of vasopressin in a rat model of trait anxiety.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Destabilization of tetraplex structures of the fragile X repeat sequence (CGG)n is mediated by homolog-conserved domains in three members of the hnRNP family.Nucleic acids research · 2004Article
- Multiple type A/B heterogeneous nuclear ribonucleoproteins (hnRNPs) can replace hnRNP A1 in mouse hepatitis virus RNA synthesis.Journal of virology · 2003Article
- Identification of S1 proteins B2, C1 and D1 as AUF1 isoforms and their major role as heterogeneous nuclear ribonucleoprotein proteins.The Biochemical journal · 2003Article
- Identification of a novel AU-rich-element-binding protein which is related to AUF1.The Biochemical journal · 2002Article
- Distinct domains in the CArG-box binding factor A destabilize tetraplex forms of the fragile X expanded sequence d(CGG)n.Nucleic acids research · 2002Article
- Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins.Nucleic acids research · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
In the present study we identified a positive transcriptional element within the rat Ha-ras promoter previously known as Ha-ras response element (HRE) and identified a trans-acting factor that binds HRE sequences in rat mammary cells. To identify the binding protein we employed sequence specific DNA affinity chromatography. Amino acid sequence analysis of the affinity-purified proteins was performed by tandem mass spectroscopy. The results unexpectedly demonstrated that in rat mammary cells CArG box-binding factor A (CBF-A) is the major protein species that bind specifically to the rat and human HRE sequences with high affinity. The affinity of CBF-A binding to HRE was significantly higher than to the CArG box described as a recognition sequence for CBF-A protein. Transient transfection assays using reporter plasmids verified that mutations within the HRE that disrupt binding of CBF-A also reduced the activity of the rat Ha-ras promoter. Despite the fact that the HRE within the Ha-ras promoter resembles a binding site for Ets transcription factors, we did not detect the binding of Ets-related proteins to the rat HRE in BICR-M1Rk cells. We further demonstrated a correlation between the presence of HRE binding activity and induction of Ha-ras mRNA expression following serum stimulation in the mammary carcinoma cell line. Taken together, our results suggest that CBF-A may play an important role in transcriptional regulation of Ha-ras promoter activity during normal mammary cell growth and carcinogenesis.
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