ArticleNucleic acids research2000
Identification of an enhancer and an alternative promoter in the first intron of the alpha-fetoprotein gene.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
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- The first enhancer in an enhancer chain safeguards subsequent enhancer-promoter contacts from a distance.Genome biology · 2019Article
- Effect of variation in ovine WFIKKN2 on growth traits appears to be gender-dependent.Scientific reports · 2015Article
- Effects of SNPs and alternative splicing within HGF gene on its expression patterns in Qinchuan cattle.Journal of animal science and biotechnology · 2015Article
- Novel kinin B₁ receptor splice variant and 5'UTR regulatory elements are responsible for cell specific B₁ receptor expression.PloS one · 2014Article
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- An intronic MYLK variant associated with inflammatory lung disease regulates promoter activity of the smooth muscle myosin light chain kinase isoform.Journal of molecular medicine (Berlin, Germany) · 2012Article
- The function of introns.Frontiers in genetics · 2012Article
- Tough beginnings: alterations in the transcriptome of cloned embryos during the first two cell cycles.Developmental biology · 2007Article
- Arabidopsis sucrose transporter AtSUC9. High-affinity transport activity, intragenic control of expression, and early flowering mutant phenotype.Plant physiology · 2007Article
- Tg(Afp-GFP) expression marks primitive and definitive endoderm lineages during mouse development.Developmental dynamics : an official publication of the American Association of Anatomists · 2006Article
- The family feud: turning off Sp1 by Sp1-like KLF proteins.The Biochemical journal · 2005Review
- Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A region in the first exon/intron of rat carnitine palmitoyltransferase Ibeta is involved in enhancement of basal transcription.The Biochemical journal · 2002Article
- Alpha-fetoprotein: A Multifaceted Player in Cancer Biology.Euroasian journal of hepato-gastroenterologyReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we have characterized a positive regulatory region located in the first intron of the alpha-fetoprotein (AFP) gene. We show that the enhancer activity of the region depends on a 44 bp sequence centered on a CACCC motif. The sequence is the target of the two zinc fingers transcription factors BKLF and YY1. The introduction of a mutation destroying the CACCC box impairs the binding of BKLF but improves that of YY1. Moreover, the mutated sequence behaves as a negative control element, suggesting that BKLF behaves as a positive factor and that YY1 is a negative one. We also demonstrate the existence of a novel, tissue-specific AFP mRNA isoform present in the yolk sac and fetal liver which initiates from an alternative promoter located approximately 100 bp downstream of the enhancer element. The transcriptional start site controlled by this new promoter (called P2), was mapped to 66 bp downstream of a TATA box. A putative AUG translation site in-frame with exon 2 of the classical gene was found 295 bp downstream of the transcription start site. Like the traditional AFP promoter (P1), the P2 promoter is active in the yolk sac and fetal liver. Embryonic stem cells with an AFP knock-in gene containing either the P2 promoter or deleted for it were isolated and comparative analysis of embryonic bodies derived from these cells suggests that the P2 promoter contributes to early expression of the AFP gene.
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