ArticleNucleic acids research2000
Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Characterization of a novel class I transcription factor A (CITFA) subunit that is indispensable for transcription by the multifunctional RNA polymerase I of Trypanosoma brucei.Eukaryotic cell · 2012Article
- Transcription by the multifunctional RNA polymerase I in Trypanosoma brucei functions independently of RPB7.Molecular and biochemical parasitology · 2011Article
- The pre-mRNA splicing machinery of trypanosomes: complex or simplified?Eukaryotic cell · 2010Review
- Spliceosomal proteomics in Trypanosoma brucei reveal new RNA splicing factors.Eukaryotic cell · 2009Article
- Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1.Nucleic acids research · 2009Article
- Evidence for a capping enzyme with specificity for the trypanosome spliced leader RNA.Molecular and biochemical parasitology · 2007Article
- Multifunctional class I transcription in Trypanosoma brucei depends on a novel protein complex.The EMBO journal · 2007Article
- Characterization of the Trypanosoma brucei cap hypermethylase Tgs1.Molecular and biochemical parasitology · 2007Article
- Spliced leader RNA gene transcription in Trypanosoma brucei requires transcription factor TFIIH.Eukaryotic cell · 2007Article
- A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei.Nucleic acids research · 2006Article
- Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination.Eukaryotic cell · 2005Article
- Characterization of a multisubunit transcription factor complex essential for spliced-leader RNA gene transcription in Trypanosoma brucei.Molecular and cellular biology · 2005Article
- Cap-binding activity of an eIF4E homolog from Leishmania.RNA (New York, N.Y.) · 2004Article
- The Trypanosoma brucei spliced leader RNA and rRNA gene promoters have interchangeable TbSNAP50-binding elements.Nucleic acids research · 2004Article
- Unconventional rules of small nuclear RNA transcription and cap modification in trypanosomatids.Gene expression · 2002Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In most eukaryotic organisms the U2 small nuclear RNA (snRNA) gene is transcribed by RNA polymerase II to generate a primary transcript with a 5' terminal 7-methylguanosine cap structure. Following nuclear export, the U2 snRNA is assembled into a core ribonucleoprotein particle (RNP). This involves binding a set of proteins that are shared by spliceosomal snRNPs to the highly conserved Sm site. Prior to nuclear import, the snRNA-(guanosine-N:2)-methyltransferase appears to interact with the core RNP and hypermethylates the cap structure to 2,2, 7-trimethylguanosine (m(3)G). In the protist parasite Trypanosoma brucei, U-snRNAs are complexed with a set of common proteins that are analogous to eukaryotic Sm antigens but do not have a highly conserved Sm sequence motif, and most U-snRNAs are synthesised by RNA polymerase III. Here, we examined the determinants for m(3)G cap formation in T.brucei by expressing mutant U2 snRNAs in vivo and assaying trimethylation and RNP assembly by immunoprecipitation. Surprisingly, these studies revealed that the Sm-analogous region is not required either for binding of the common proteins or for cap trimethylation. Furthermore, except for the first 24 nt which are part of the U2 promoter, the U2 coding region could be substituted or deleted without affecting cap trimethylation.
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