ArticleJournal of virology2000
In vitro and in vivo characterization of a murine cytomegalovirus with a transposon insertional mutation at open reading frame M43.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- An OralPathogens (Basel, Switzerland) · 2025Article
- Antiviral Immune Responses Against Murine Cytomegalovirus Induced by an OralMicroorganisms · 2025Article
- Oral Vaccination with AttenuatedPathogens (Basel, Switzerland) · 2025Article
- Article
- Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes.Journal of virology · 2011Article
- Conditional gene expression systems to study herpesvirus biology in vivo.Medical microbiology and immunology · 2008Review
- Characterization of the murine cytomegalovirus m136 gene.Virus genes · 2007Article
- Murine cytomegalovirus encodes a stable intron that facilitates persistent replication in the mouse.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Complex formation among murine cytomegalovirus US22 proteins encoded by genes M139, M140, and M141.Journal of virology · 2005Article
- Murine cytomegalovirus with a transposon insertional mutation at open reading frame m155 is deficient in growth and virulence in mice.Journal of virology · 2004Article
- Murine cytomegalovirus with a transposon insertional mutation at open reading frame M35 is defective in growth in vivo.Journal of virology · 2003Article
- Role of murine cytomegalovirus US22 gene family members in replication in macrophages.Journal of virology · 2003Article
- Article
- Products of US22 genes M140 and M141 confer efficient replication of murine cytomegalovirus in macrophages and spleen.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently generated a pool of murine cytomegalovirus (MCMV) mutants by using a Tn3-based transposon mutagenesis approach. In this study, one of the MCMV mutants, RvM43, which contained the transposon inserted in open reading frame M43, was characterized. Our results provide the first direct evidence to suggest that M43 is not essential for viral replication in vitro in NIH 3T3 cells. Moreover, RvM43 exhibited a titer similar to that of the wild-type virus in the lungs, livers, spleens, and kidneys of both BALB/c and SCID mice and was as virulent as the wild-type virus in killing SCID mice that had been intraperitoneally infected with the viruses. In contrast, titers of the mutant virus in the salivary glands of the infected animals at 21 days postinfection were significantly (100 to 1,000-fold) lower than those of the wild-type virus and a rescued virus that restored the M43 region and its expression. Thus, M43 appears to be not essential for viral growth in vivo in the lungs, livers, spleens, and kidneys of infected animals and is also dispensable for virulence in killing SCID mice. Moreover, our results suggest that M43 is an MCMV determinant for growth in the salivary glands. Studies of viral genes required for replication in the salivary glands are important in understanding the mechanism of viral tropism for the salivary glands and shedding in saliva, which is believed to be one of the major routes of CMV transmission among healthy human populations.
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