ArticleRNA (New York, N.Y.)2000
Coupled in vitro synthesis and splicing of RNA polymerase II transcripts.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- mRNA splicing is modulated by intronic microRNAs.iScience · 2023Article
- Methodologies for studying the spliceosome's RNA dynamics with single-molecule FRET.Methods (San Diego, Calif.) · 2017Review
- FUS functions in coupling transcription to splicing by mediating an interaction between RNAP II and U1 snRNP.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Primary microRNA processing is functionally coupled to RNAP II transcription in vitro.Scientific reports · 2015Article
- In vitro systems for coupling RNAP II transcription to splicing and polyadenylation.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Introduction to cotranscriptional RNA splicing.Methods in molecular biology (Clifton, N.J.) · 2014Article
- The RNA polymerase II CTD coordinates transcription and RNA processing.Genes & development · 2012Review
- Small-scale nuclear extracts for functional assays of gene-expression machineries.Journal of visualized experiments : JoVE · 2012Article
- RNA Polymerase II Elongation at the Crossroads of Transcription and Alternative Splicing.Genetics research international · 2011Article
- A model in vitro system for co-transcriptional splicing.Nucleic acids research · 2010Article
- Cotranscriptional exon skipping in the genotoxic stress response.Nature structural & molecular biology · 2010Article
- TLS inhibits RNA polymerase III transcription.Molecular and cellular biology · 2010Article
- An Alu-derived intronic splicing enhancer facilitates intronic processing and modulates aberrant splicing in ATM.Nucleic acids research · 2009Article
- The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase.The Journal of biological chemistry · 2009Article
- Mir-434-5p mediates skin whitening and lightening.Clinical, cosmetic and investigational dermatology · 2008Article
- Cotranscriptional splicing potentiates the mRNA production from a subset of estradiol-stimulated genes.Molecular and cellular biology · 2008Article
- Functional coupling of last-intron splicing and 3'-end processing to transcription in vitro: the poly(A) signal couples to splicing before committing to cleavage.Molecular and cellular biology · 2008Article
- Concurrent splicing and transcription are not sufficient to enhance splicing efficiency.RNA (New York, N.Y.) · 2007Article
- A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional.Molecular cell · 2006Article
- Human transcription elongation factor CA150 localizes to splicing factor-rich nuclear speckles and assembles transcription and splicing components into complexes through its amino and carboxyl regions.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compelling in vivo studies suggest a tight functional linkage between RNA polymerase II transcription and premessenger RNA splicing. At present, the specific interactions involved in this coupling are poorly understood and deserve investigation. To this end, we developed an in vitro system that permits study of coupled transcription and splicing. Transcripts generated by RNA polymerase II were accurately and efficiently spliced under reaction conditions that permitted both transcription and splicing to occur simultaneously. The splicing of RNA-polymerase-II-driven transcripts was accelerated relative to that of the same transcripts driven by T7 RNA polymerase. Moreover, the product of exon ligation was found associated with the DNA template in reactions driven by RNA polymerase II. These two findings indicate that transcription and splicing were coupled in the in vitro system driven by RNA polymerase II, and suggest that this system will be useful for the biochemical study of this coupling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.