ArticleRNA (New York, N.Y.)2000
Effect of 3' terminal adenylic acid residue on the uridylation of human small RNAs in vitro and in frog oocytes.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Meta-analysis of small RNA-sequencing errors reveals ubiquitous post-transcriptional RNA modifications.Nucleic acids research · 2009Pooled it
- Widespread mono- and oligoadenylation direct small noncoding RNA maturation versus degradation fates.The EMBO journal · 2026Article
- YTHDF2 regulates self non-coding RNA metabolism to control inflammation and tumorigenesis.Nature communications · 2025Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Truncated forms of U2 snRNA (U2-tfs) are shunted toward a novel uridylylation pathway that differs from the degradation pathway for U1-tfs.RNA biology · 2018Article
- An Improved microRNA Annotation of the Canine Genome.PloS one · 2016Article
- Polyuridylation in Eukaryotes: A 3'-End Modification Regulating RNA Life.BioMed research international · 2015Review
- Poly(A) polymerase (PAP) diversity in gene expression--star-PAP vs canonical PAP.FEBS letters · 2014Review
- The panorama of miRNA-mediated mechanisms in mammalian cells.Cellular and molecular life sciences : CMLS · 2014Review
- A critical switch in the enzymatic properties of the Cid1 protein deciphered from its product-bound crystal structure.Nucleic acids research · 2014Article
- Role of the Trypanosoma brucei HEN1 family methyltransferase in small interfering RNA modification.Eukaryotic cell · 2014Article
- 3' terminal diversity of MRP RNA and other human noncoding RNAs revealed by deep sequencing.BMC molecular biology · 2013Article
- A genome-wide survey of microRNA truncation and 3' nucleotide addition events in larch (Larix leptolepis).Planta · 2013Article
- Uridylation of miRNAs by hen1 suppressor1 in Arabidopsis.Current biology : CB · 2012Article
- Regulation of small RNA stability: methylation and beyond.Cell research · 2012Review
- Article
- MicroRNA assassins: factors that regulate the disappearance of miRNAs.Nature structural & molecular biology · 2010Review
- Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2.Genes & development · 2009Article
- A novel gene expression pathway regulated by nuclear phosphoinositides.Advances in enzyme regulation · 2009Review
- 3' Terminal oligo U-tract-mediated stimulation of decapping.RNA (New York, N.Y.) · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
It is known that several small RNAs including human and Xenopus signal recognition particle (SRP) RNA, U2 small nuclear RNA (snRNA) and 7SK RNAs are posttranscriptionally adenylated, whereas U6 snRNA and ribosomal 5S RNA are posttranscriptionally uridylated on their 3' ends. In this study, we provide evidence that a small fraction of U6 snRNA and 5S ribosomal RNA molecules from human as well as Xenopus oocytes contain a single posttranscriptionally added adenylic acid residue on their 3' ends. These data show that U6 snRNA and 5S rRNAs are posttranscriptionally modified on their 3' ends by both uridylation and adenylation. Although the SRP RNA, 7SK RNA, 5S RNA, and U6 snRNA with the uridylic acid residue on their 3' ends were readily uridylated, all these RNAs with posttranscriptionally added adenylic acid residue on their 3' ends were not uridylated in vitro, or when U6 snRNA with 3' A(OH) was injected into Xenopus oocytes. These results show that the presence of a single posttranscriptionally added adenylic acid residue on the 3' end of SRP RNA, U6 snRNA, 5S rRNA, or 7SK RNA prevents 3' uridylation. These data also show that adenylation and uridylation are two competing processes that add nucleotides on the 3' end of some small RNAs and suggest that one of the functions of the 3' adenylation may be to negatively affect the 3' uridylation of small RNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.