ReviewPlant molecular biology2000
Plants as bioreactors for protein production: avoiding the problem of transgene silencing.
Review in Plant molecular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epigenomics as Potential Tools for Enhancing Magnitude of Breeding Approaches for Developing Climate Resilient Chickpea.Frontiers in genetics · 2022Pooled it
- Characterization ofSynthetic and systems biotechnology · 2027Article
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- Identification and application of bioparts for plant synthetic biology.Molecules and cells · 2025Review
- Article
- Characterization of the VOC Promoter That Is Active Under Low-Salinity Conditions in the DiatomMarine drugs · 2025Article
- Building a pipeline to identify and engineer constitutive and repressible promoters.Quantitative plant biology · 2023Article
- Energetic considerations for engineering novel biochemistries in photosynthetic organisms.Frontiers in plant science · 2023Review
- Leaf layer-based transcriptome profiling for discovery of epidermal-selective promoters in Medicago truncatula.Planta · 2022Article
- Benchmarking Intrinsic Promoters and Terminators for Plant Synthetic Biology Research.Biodesign research · 2022Article
- Overexpression of Douglas-FirPlants (Basel, Switzerland) · 2021Article
- Analyzing the action of evolutionarily conserved modules on HMW-GS 1Ax1 promoter activity.Plant molecular biology · 2020Article
- Isolation and characterization of a novel seed-specific promoter from peanut (Arachis hypogaea L.).Molecular biology reports · 2019Article
- Article
- Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.Plant biotechnology journal · 2018Article
- Expression of a novel bi-directional Brassica napus promoter in soybean.Transgenic research · 2017Article
- Investigating Triticeae anther gene promoter activity in transgenic Brachypodium distachyon.Planta · 2017Article
- The pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in Arabidopsis thaliana plants.World journal of microbiology & biotechnology · 2016Article
- Article
- Identification of a 467 bp Promoter of Maize Phosphatidylinositol Synthase Gene (ZmPIS) Which Confers High-Level Gene Expression and Salinity or Osmotic Stress Inducibility in Transgenic Tobacco.Frontiers in plant science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants are particularly attractive as large-scale production systems for proteins intended for therapeutical or industrial applications: they can be grown easily and inexpensively in large quantities that can be harvested and processed with the available agronomic infrastructures. The effective use of plants as bioreactors depends on the possibility of obtaining high protein accumulation levels that are stable during the life cycle of the transgenic plant and in subsequent generations. Silencing of the introduced transgenes has frequently been observed in plants, constituting a major commercial risk and hampering the general economic exploitation of plants as protein factories. Until now, the most efficient strategy to avoid transgene silencing involves careful design of the transgene construct and thorough analysis of transformants at the molecular level. Here, we focus on different aspects of the generation of transgenic plants intended for protein production and on their influence on the stability of heterologous gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.