ArticleThe Biochemical journal2000
Cloning and functional expression of a gene encoding a vacuolar-type proton-translocating pyrophosphatase from Trypanosoma cruzi.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes.Microbiology and molecular biology reviews : MMBR · 2024Review
- New insights into the role of acidocalcisomes in trypanosomatids.The Journal of eukaryotic microbiology · 2022Review
- The flip side of theThe Journal of biological chemistry · 2019Article
- Characterization and expression analyses of the H⁺-pyrophosphatase gene in rye.Journal of genetics · 2016Article
- Acidocalcisomes of eukaryotes.Current opinion in cell biology · 2016Review
- Generation and Identification of Peptide-Based Monoclonal Antibodies Against Vacuolar Proton Pyrophosphatase of Toxoplasma gondii.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2015Article
- Identification and characterization of an ecto-pyrophosphatase activity in intact epimastigotes of Trypanosoma rangeli.PloS one · 2014Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Acidocalcisomes.Cell calcium · 2011Review
- Na+-translocating membrane pyrophosphatases are widespread in the microbial world and evolutionarily precede H+-translocating pyrophosphatases.The Journal of biological chemistry · 2011Article
- The role of acidocalcisomes in the stress response of Trypanosoma cruzi.Advances in parasitology · 2011Article
- Characterization of a novel organelle in Toxoplasma gondii with similar composition and function to the plant vacuole.Molecular microbiology · 2010Article
- The acidocalcisome as a target for chemotherapeutic agents in protozoan parasites.Current pharmaceutical design · 2008Review
- Down-regulation of the trypanosomatid signal recognition particle affects the biogenesis of polytopic membrane proteins but not of signal peptide-containing proteins.Eukaryotic cell · 2007Article
- Functional complementation of yeast cytosolic pyrophosphatase by bacterial and plant H+-translocating pyrophosphatases.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Secretory pathway of trypanosomatid parasites.Microbiology and molecular biology reviews : MMBR · 2002Review
- Identification and analysis of proton-translocating pyrophosphatases in the methanogenic archaeon Methansarcina mazei.Archaea (Vancouver, B.C.) · 2002Article
- The role of acidocalcisomes in parasitic protists.The Journal of eukaryotic microbiologyReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Acidocalcisomes are acidic Ca(2+)-storage organelles found in trypanosomatids that are similar to organelles known historically as volutin granules. Acidification of these organelles is driven in part by a vacuolar H(+)-pyrophosphatase (V-H(+)-PPase), an enzyme that is also present in plant vacuoles and in some bacteria. Here, we report the cloning and sequencing of a gene encoding the acidocalcisomal V-H(+)-PPase of Trypanosoma cruzi. The protein (T. cruzi pyrophosphatase, TcPPase) predicted from the nucleotide sequence of the gene has 816 amino acids and a molecular mass of 85 kDa. Several sequence motifs found in plant V-H(+)-PPases were present in TcPPase, explaining its sensitivity to N-ethylmaleimide and N,N'-dicyclohexylcarbodi-imide. Heterologous expression of the cDNA encoding TcPPase in the yeast Saccharomyces cerevisiae produced a functional enzyme. Phylogenetic analysis of the available V-H(+)-PPase sequences indicates that TcPPase is nearer to the vascular plant cluster and the branch containing Chara, a precursor to land plants, than to any of the other pyrophosphatase sequences included in the analysis. The apparent lack of such a V-H(+)-PPase in mammalian cells may provide a target for the development of new drugs.
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