ArticleThe Biochemical journal2000
p53 protein oxidation in cultured cells in response to pyrrolidine dithiocarbamate: a novel method for relating the amount of p53 oxidation in vivo to the regulation of p53-responsive genes.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- Deciphering the safeguarding role of cysteine residues in p53 against HCommunications chemistry · 2025Article
- A Novel Peptide Reagent for Investigating Disulfide-Coupled Folding Intermediates of Mid-Size Proteins.Molecules (Basel, Switzerland) · 2023Article
- RBBP4 dysfunction reshapes the genomic landscape of H3K27 methylation and acetylation and disrupts gene expression.G3 (Bethesda, Md.) · 2022Article
- Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate.Oxidative medicine and cellular longevity · 2021Article
- Revisiting cancer hallmarks: insights from the interplay between oxidative stress and non-coding RNAs.Molecular biomedicine · 2020Review
- Cytotoxic phenanthroline derivatives alter metallostasis and redox homeostasis in neuroblastoma cells.Oncotarget · 2018Article
- SMAD signaling and redox imbalance cooperate to induce prostate cancer cell dormancy.Cell cycle (Georgetown, Tex.) · 2015Article
- Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Nanomaterials and nanotechnology for skin tissue engineering.International journal of burns and trauma · 2012Article
- Purification of reversibly oxidized proteins (PROP) reveals a redox switch controlling p38 MAP kinase activity.PloS one · 2010Article
- Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli.Microbial cell factories · 2010Article
- Targeted quantitation of site-specific cysteine oxidation in endogenous proteins using a differential alkylation and multiple reaction monitoring mass spectrometry approach.Molecular & cellular proteomics : MCP · 2010Article
- 15-Deoxy-Delta(12,14)-prostaglandin J(2) stabilizes, but functionally inactivates p53 by binding to the cysteine 277 residue.Oncogene · 2010Article
- Novel thioredoxin-related transmembrane protein TMX4 has reductase activity.The Journal of biological chemistry · 2010Article
- Effect of thioredoxin deletion and p53 cysteine replacement on human p53 activity in wild-type and thioredoxin reductase null yeast.Biochemistry · 2009Article
- Reconstitution of the mia40-erv1 oxidative folding pathway for the small tim proteins.Molecular biology of the cell · 2009Article
- Glutathionylation of the pro-apoptotic protein p53 in Alzheimer's disease brain: implications for AD pathogenesis.Neurochemical research · 2009Article
- Contribution of the HEDJ/ERdj3 cysteine-rich domain to substrate interactions.Archives of biochemistry and biophysics · 2007Article
- Human p53 is inhibited by glutathionylation of cysteines present in the proximal DNA-binding domain during oxidative stress.Biochemistry · 2007Article
- p53 and p73 display common and distinct requirements for sequence specific binding to DNA.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel method was developed to determine the oxidation status of proteins in cultured cells. Methoxy-polyethylene glycol-maleimide MW 2000 (MAL-PEG) was used to covalently tag p53 protein that was oxidized at cysteine residues in cultured cells. Treatment of MCF7 breast cancer cells with pyrrolidine dithiocarbamate (PDTC), a metal chelator, resulted in a minimum of 25% oxidation of p53. The oxidized p53 had an average of one cysteine residue oxidized per p53 protein molecule. The effect of PDTC treatment on downstream components of the p53 signal-transduction pathway was tested. PDTC treatment prevented actinomycin D-mediated up-regulation of two p53 effector gene products, murine double minute clone 2 oncoprotein and p21(WAF1/CIP1) (where WAF1 corresponds to wild-type p53-activated fragment 1 and CIP1 corresponds to cyclin-dependent kinase-interacting protein 1). Actinomycin D treatment led to accumulation of p53 protein in the nucleus. However, when cells were simultaneously treated with PDTC and actinomycin D, p53 accumulated in both the nucleus and the cytoplasm. The data indicate that an average of one cysteine residue per p53 protein molecule is highly sensitive to oxidation and that p53 can be efficiently oxidized by PDTC in cultured cells. PDTC-mediated oxidation of p53 correlates with altered p53 subcellular localization and reduced activation of p53 downstream effector genes. The novel method for detecting protein oxidation detailed in the present study may be used to determine the oxidation status of specific proteins in cells.
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