Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen.

R R Duncan, D K Apps, M P Learmonth, M J Shipston, R H Chow

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Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.1field-weighted citation impact, top 53% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions.Molecular biology of the cell · 2005
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

R R DuncanMembrane Biology Group, University of Edinburgh, Medical School, Department of Biomedical Sciences, Edinburgh EH8 9AG, Scotland, UK. rory.duncan@ed.ac.uk

D K Apps

M P Learmonth

M J Shipston

R H Chow

University of Edinburgh · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2alpha nor DOC2beta is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial complex III core protein 2.

Indexed as

Adrenal MedullaAnimalsAntibodies, MonoclonalAntigensBrainCalcium-Binding ProteinsCattleChromaffin CellsCross ReactionsImmune SeraMiceMitochondriaMolecular WeightNerve Tissue ProteinsProtein BiosynthesisReverse Transcriptase Polymerase Chain ReactionAntibodies, MonoclonalAntigensCalcium-Binding ProteinsDoc2a protein, mouseImmune SeraNerve Tissue ProteinsRNA, Messenger

Identifiers

PMID10998344

DOI10.1042/0264-6021:3510033

PMCPMC1221332

OpenAlexW2005642573

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.