ArticleFolia microbiologica1999
Use of the 6-methylsalicylic-acid-synthase gene as a discriminating marker between Aspergillus terreus and Aspergillus flavipes.
Article in Folia microbiologica, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Primer R108 performs best in the RAPD strain typing of three Aspergillus species frequently isolated from patients.Folia microbiologica · 2006Article
- Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In nineteen pathogenic and saprophytic isolates denoted as Aspergillus terreus the presence and restriction pattern of the gene pksM (6-methylsalicylic acid synthase) was determined. Five patterns (A-E) were found and in three isolates the gene was missing. The RAPD (Random Amplified Polymorphic DNA) patterns with three primers were analyzed. The strains with pksM pattern possibly derived from the most common pattern A by single mutation (patterns B-D) were also related by RAPD. Among them, three clades were found. The first one contained saprophytes from Asia, the other two clades contained both European and American pathogens and each of them one saprophyte. The sequences of rDNA region containing 5.8S rDNA and spacers ITS1 and ITS2 were established for representatives of each group and the strains missing the pksM gene. The isolates possessing pksM (although with different restriction patterns) grouped as A. terreus group, whereas the isolates lacking this gene were close to Fennellia flavipes.
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Registered trials
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