ArticleProceedings of the National Academy of Sciences of the United States of America2000
Glutathione biosynthesis in Arabidopsis trichome cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 181 citations in OpenAlex.
- Nematicidal and plant growth-promoting potential of the cell-free culture supernatant of Bacillus megaterium in tomato under Meloidogyne incognita infection: an in vitro and in vivo study.Archives of microbiology · 2026Article
- CRISPR-Cas9-mediated editing ofGM crops & food · 2025Article
- Beyond defense: microbial modifications of plant specialized metabolites alter and expand their ecological functions.The New phytologist · 2025Review
- Ameliorative effect of rhizobacteria Bradyrhizobium japonicum on antioxidant enzymes, cell viability and biochemistry in tomato plant under nematode stress.Scientific reports · 2025Article
- Comprehensive Analysis of the OASTL Gene Family in Potato (International journal of molecular sciences · 2024Article
- The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration.Quantitative plant biology · 2024Article
- An Approach to Evaluate Pb Tolerance and Its Removal Mechanisms byJournal of fungi (Basel, Switzerland) · 2023Article
- Micro-Evolutionary Processes inInternational journal of molecular sciences · 2023Review
- Review
- Amelioration of Chromium-Induced Oxidative Stress by Combined Treatment of Selected Plant-Growth-Promoting Rhizobacteria and EarthwormsFrontiers in microbiology · 2022Article
- Article
- Amelioration of Chlorpyrifos-Induced Toxicity inBiomolecules · 2021Article
- The Leaf Wettability of Various Potato Cultivars.Plants (Basel, Switzerland) · 2020Article
- Seed Priming with Jasmonic Acid Counteracts Root Knot Nematode Infection in Tomato by Modulating the Activity and Expression of Antioxidative Enzymes.Biomolecules · 2020Article
- Do Specialized Cells Play a Major Role in Organic Xenobiotic Detoxification in Higher Plants?Frontiers in plant science · 2020Article
- Accumulation, morpho-physiological and oxidative stress induction by single and binary treatments of fluoride and low molecular weight phthalates in Spirodela polyrhiza L. Schleiden.Scientific reports · 2019Article
- In-situ localization and biochemical analysis of bio-molecules reveals Pb-stress amelioration in Brassica juncea L. by co-application of 24-Epibrassinolide and Salicylic Acid.Scientific reports · 2019Article
- Decipher the Molecular Response of Plant Single Cell Types to Environmental Stresses.BioMed research international · 2016Review
- OsTCP19 influences developmental and abiotic stress signaling by modulating ABI4-mediated pathways.Scientific reports · 2015Article
- Possible roles of plant sulfurtransferases in detoxification of cyanide, reactive oxygen species, selected heavy metals and arsenate.Molecules (Basel, Switzerland) · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis thaliana, trichome cells are specialized unicellular structures with uncertain functions. Based on earlier observations that one of the genes involved in cysteine biosynthesis (Atcys-3A) is highly expressed in trichomes, we have extended our studies in trichome cells to determine their capacity for glutathione (GSH) biosynthesis. First, we have analyzed by in situ hybridization the tissue-specific expression of the genes Atcys-3A and sat5, which encode O-acetylserine(thio)lyase (OASTL) and serine acetyltransferase (SAT), respectively, as well as gsh1 and gsh2, which encode gamma-glutamylcysteine synthetase and glutathione synthetase, respectively. The four genes are highly expressed in leaf trichomes of Arabidopsis, and their mRNA accumulate to high levels. Second, we have directly measured cytoplasmic GSH concentration in intact cells by laser-scanning microscopy after labeling with monochlorobimane as a GSH-specific probe. From these measurements, cytosolic GSH concentrations of 238+/-25, 80+/-2, and 144+/-19 microM were estimated for trichome, basement, and epidermal cells, respectively. Taking into account the volume of the cells measured using stereological techniques, the trichomes have a total GSH content more than 300-fold higher than the basement and epidermal cells. Third, after NaCl treatment, GSH biosynthesis is markedly decreased in trichomes. Atcys-3A, sat5, gsh1, and gsh2 mRNA levels show a decrease in transcript abundance, and [GSH](cyt) is reduced to 47+/-5 microM. These results suggest the important physiological significance of trichome cells related to GSH biosynthesis and their possible role as a sink during detoxification processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.