ArticleThe Journal of cell biology2000
Calcium regulates the association between mitochondria and a smooth subdomain of the endoplasmic reticulum.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 173 citations in OpenAlex.
- ER proteostasis meets mitochondrial function: contact sites as hubs of communication and therapeutic targets.The FEBS journal · 2026Review
- Mitochondria‑endoplasmic reticulum contact site nexus: Molecular integration, disease pathogenesis and therapeutic opportunities (Review).International journal of molecular medicine · 2026Review
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- A tripartite organelle platform links growth factor receptor signaling to mitochondrial metabolism.Nature communications · 2024Article
- Review
- COL-3-Induced Molecular and Ultrastructural Alterations in K562 Cells.Journal of personalized medicine · 2022Article
- Autophagy in the Neuronal Ceroid Lipofuscinoses (Batten Disease).Frontiers in cell and developmental biology · 2022Review
- Mitochondria-Associated Endoplasmic Reticulum Membranes: Inextricably Linked with Autophagy Process.Oxidative medicine and cellular longevity · 2022Review
- Paranodal Axoglial Junctions, an Essential Component in Axonal Homeostasis.Frontiers in cell and developmental biology · 2022Review
- RETREG1/FAM134B mediated autophagosomal degradation of AMFR/GP78 and OPA1 -a dual organellar turnover mechanism.Autophagy · 2021Article
- Article
- Review
- Mitochondria-Associated ER Membranes - The Origin Site of Autophagy.Frontiers in cell and developmental biology · 2020Review
- Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.Neurology (E-Cronicon) · 2019Article
- Membrane-Deformation Ability of ANKHD1 Is Involved in the Early Endosome Enlargement.iScience · 2019Article
- Link between the unfolded protein response and dysregulation of mitochondrial bioenergetics in Alzheimer's disease.Cellular and molecular life sciences : CMLS · 2019Article
- Endoplasmic Reticulum-Mitochondrial Contactology: Structure and Signaling Functions.Trends in cell biology · 2018Review
- ER-mitochondria signaling in Parkinson's disease.Cell death & disease · 2018Review
- Mitochondria-associated ER membranes (MAMs) and lysosomal storage diseases.Cell death & disease · 2018Review
- Emerging molecular mechanisms in chemotherapy: CaCell death & disease · 2018Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Association between the ER and mitochondria has long been observed, and the formation of close contacts between ER and mitochondria is necessary for the ER-mediated sequestration of cytosolic calcium by mitochondria. Autocrine motility factor receptor (AMF-R) is a marker for a smooth subdomain of the ER, shown here by confocal microscopy to be distinct from, yet closely associated with the calnexin- or calreticulin-labeled ER. By EM, smooth ER AMF-R tubules exhibit direct interactions with mitochondria, identifying them as a mitochondria-associated smooth ER subdomain. In digitonin-permeabilized MDCK cells, the addition of rat liver cytosol stimulates the dissociation of smooth ER and mitochondria under conditions of low calcium. Using BAPTA chelators of various affinities and CaEGTA buffers of defined free Ca(2+) concentrations and quantitative confocal microscopy, we show that free calcium concentrations <100 nM favor dissociation, whereas those >1 microM favor close association between these two organelles. Therefore, we describe a cellular mechanism that facilitates the close association of this smooth ER subdomain and mitochondria when cytosolic free calcium rises above physiological levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.