ArticleThe Journal of cell biology2000
Exclusion of golgi residents from transport vesicles budding from Golgi cisternae in intact cells.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Models of Intracellular Transport: Contradictions and Current Understanding.Sub-cellular biochemistry · 2026Review
- Mechanistic insights into cargo sorting and export from the Golgi apparatus.Nature reviews. Molecular cell biology · 2025Review
- An Overview of Protein Secretion in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Passive diffusion accounts for the majority of intracellular nanovesicle transport.Life science alliance · 2024Article
- Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra-Golgi recycling vesicles.Traffic (Copenhagen, Denmark) · 2023Article
- Integrating intracellular nanovesicles into integrin trafficking pathways and beyond.Cellular and molecular life sciences : CMLS · 2022Review
- On the nature of the plant ER exit sites.Frontiers in plant science · 2022Article
- Tumor protein D54 defines a new class of intracellular transport vesicles.The Journal of cell biology · 2020Article
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
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- ARF1 and SAR1 GTPases in endomembrane trafficking in plants.International journal of molecular sciences · 2013Review
- A three-stage model of Golgi structure and function.Histochemistry and cell biology · 2013Review
- The dynamics of engineered resident proteins in the mammalian Golgi complex relies on cisternal maturation.The Journal of cell biology · 2013Article
- An organelle gatekeeper function for Caenorhabditis elegans UNC-16 (JIP3) at the axon initial segment.Genetics · 2013Article
- Alcohol induces Golgi fragmentation in differentiated PC12 cells by deregulating Rab1-dependent ER-to-Golgi transport.Histochemistry and cell biology · 2012Article
- The kiss-and-run model of intra-Golgi transport.International journal of molecular sciences · 2012Article
- Models for Golgi traffic: a critical assessment.Cold Spring Harbor perspectives in biology · 2011Review
- Following the fate in vivo of COPI vesicles generated in vitro.Traffic (Copenhagen, Denmark) · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A central feature of cisternal progression/maturation models for anterograde transport across the Golgi stack is the requirement that the entire population of steady-state residents of this organelle be continuously transported backward to earlier cisternae to avoid loss of these residents as the membrane of the oldest (trans-most) cisterna departs the stack. For this to occur, resident proteins must be packaged into retrograde-directed transport vesicles, and to occur at the rate of anterograde transport, resident proteins must be present in vesicles at a higher concentration than in cisternal membranes. We have tested this prediction by localizing two steady-state residents of medial Golgi cisternae (mannosidase II and N-acetylglucosaminyl transferase I) at the electron microscopic level in intact cells. In both cases, these abundant cisternal constituents were strongly excluded from buds and vesicles. This result suggests that cisternal progression takes place substantially more slowly than most protein transport and therefore is unlikely to be the predominant mechanism of anterograde movement.
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