ArticleGenes & development2000
A nonproteolytic function of the proteasome is required for the dissociation of Cdc2 and cyclin B at the end of M phase.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 67 citations in OpenAlex.
- Proteasomal subunit depletions differentially affect germline integrity inFrontiers in cell and developmental biology · 2022Article
- Effects of Ferrocenyl 4-(Imino)-1,4-Dihydro-quinolines onInternational journal of molecular sciences · 2020Article
- MPF-based meiotic cell cycle control: Half a century of lessons from starfish oocytes.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Functions of the proteasome on chromatin.Biomolecules · 2014Review
- Calcium signaling and meiotic exit at fertilization in Xenopus egg.International journal of molecular sciences · 2014Review
- Regulated protein turnover: snapshots of the proteasome in action.Nature reviews. Molecular cell biology · 2014Review
- Cdc25B phosphatase participates in maintaining metaphase II arrest in mouse oocytes.Molecules and cells · 2013Article
- Cdc25A activity is required for the metaphase II arrest in mouse oocytes.Journal of cell science · 2013Article
- Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Ubiquitin and proteasomes in transcription.Annual review of biochemistry · 2012Review
- Canonical and Alternative Pathways in Cyclin-Dependent Kinase 1/Cyclin B Inactivation upon M-Phase Exit in Xenopus laevis Cell-Free Extracts.Enzyme research · 2011Article
- The APC/C recruits cyclin B1-Cdk1-Cks in prometaphase before D box recognition to control mitotic exit.The Journal of cell biology · 2010Article
- The Mycobacterium tuberculosis proteasome active site threonine is essential for persistence yet dispensable for replication and resistance to nitric oxide.PLoS pathogens · 2010Article
- Gamma-tubulin regulates the anaphase-promoting complex/cyclosome during interphase.The Journal of cell biology · 2010Article
- Targeting proteins for degradation.Nature chemical biology · 2009Review
- The proteasome makes sense of mixed signals.Nature chemical biology · 2009Article
- Substrate selection by the proteasome during degradation of protein complexes.Nature chemical biology · 2009Article
- Isolation, characterization, and genetic complementation of a cellular mutant resistant to retroviral infection.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Cell-cycle-dependent Xenopus TRF1 recruitment to telomere chromatin regulated by Polo-like kinase.The EMBO journal · 2006Article
- Negative regulation of geminin by CDK-dependent ubiquitination controls replication licensing.Cell cycle (Georgetown, Tex.) · 2004Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inactivation of cyclin B-Cdc2 kinase at the exit from M phase depends on the specific proteolysis of the cyclin B subunit, whereas the Cdc2 subunit remains present at nearly constant levels throughout the cell cycle. It is unknown how Cdc2 escapes degradation when cyclin B is destroyed. In Xenopus egg extracts that reproduce the exit from M phase in vitro, we have found that dissociation of the cyclin B-Cdc2 complex occurred under conditions where cyclin B was tethered to the 26S proteasome but not yet degraded. The dephosphorylation of Thr 161 on Cdc2 was unlikely to be necessary for the dissociation of the two subunits. However, the dissociation was dependent on the presence of a functional destruction box in cyclin B. Cyclin B ubiquitination was also, by itself, not sufficient for separation of Cdc2 and cyclin B. The 26S proteasome, but not the 20S proteasome, was capable of dissociating the two subunits. These results indicate that the cyclin B and Cdc2 subunits are separated by the proteasome through a mechanism that precedes proteolysis of cyclin B and is independent of proteolysis. As a result, cyclin B levels decrease on exit from M phase but Cdc2 levels remain constant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.