ArticleThe Journal of experimental medicine2000
Human endothelial cells regulate survival and proliferation of human mast cells.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 93 citations in OpenAlex.
- Mast cells: key players in digestive system tumors and their interactions with immune cells.Cell death discovery · 2025Review
- Control of myeloid cell functions by nociceptors.Frontiers in immunology · 2023Review
- Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation.Stem cells international · 2018Article
- Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B.Nature communications · 2017Article
- Mast cells in airway diseases and interstitial lung disease.European journal of pharmacology · 2016Review
- Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells.Respiratory research · 2015Article
- Dendritic cell c-kit signaling and adaptive immunity: implications for the upper airways.Current opinion in allergy and clinical immunology · 2014Review
- Cancer cells regulate biomechanical properties of human microvascular endothelial cells.The Journal of biological chemistry · 2011Article
- Signaling of c-kit in dendritic cells influences adaptive immunity.Annals of the New York Academy of Sciences · 2010Review
- Physiological and pathophysiological functions of intestinal mast cells.Seminars in immunopathology · 2009Review
- Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Breakdown of the endothelial barrier function in tumor cell transmigration.Biophysical journal · 2008Article
- Role of the endothelium during tumor cell metastasis: is the endothelium a barrier or a promoter for cell invasion and metastasis?Journal of biophysics (Hindawi Publishing Corporation : Online) · 2008Article
- Eosinophil activation of fibroblasts from chronic allergen-induced disease utilizes stem cell factor for phenotypic changes.The American journal of pathology · 2008Article
- Article
- Signaling pathways influencing SLF and c-kit-mediated survival and proliferation.Immunologic research · 2006Review
- Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Expansion of cord blood CD34+ hematopoietic progenitor cells in coculture with autologous umbilical vein endothelial cells (HUVEC) is superior to cytokine-supplemented liquid culture.Bone marrow transplantation · 2005Article
- Neurofibromin-deficient Schwann cells secrete a potent migratory stimulus for Nf1+/- mast cells.The Journal of clinical investigation · 2003Article
- Mast cells in vulnerable atherosclerotic plaques--a view to a kill.Journal of cellular and molecular medicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mast cells (MCs) are immunoregulatory and inflammatory tissue cells preferentially located around blood vessels. Since endothelial cells have been suggested to regulate MC functions, we analyzed MC-endothelial cell interactions in vitro by performing coculture experiments with purified human intestinal MCs and human umbilical vein endothelial cells (HUVECs). We found that HUVECs provide signals allowing MCs to survive for at least 3 wk and to proliferate without addition of cytokines; otherwise all MCs died. HUVEC-dependent MC proliferation was more pronounced than that induced by stem cell factor (SCF), known to act as an MC growth factor both in vitro and in vivo. After coculture with HUVECs, most MCs were of the tryptase and chymase double-positive phenotype (MC(TC)). Transwell experiments suggested that the HUVECs' effects on MCs are not mediated by soluble factors. HUVEC-dependent MC adhesion and proliferation were inhibited by neutralizing antibodies directed against SCF and vascular cell adhesion molecule (VCAM)-1 expressed on HUVECs, and c-kit and very late antigen 4 (VLA-4) on MCs. The data suggest that two mechanisms (membrane-bound SCF/c-kit and VCAM-1/VLA-4) are involved in human MC-endothelial cell interactions. In conclusion, our study provides evidence that endothelial cells regulate MC survival and preferentially support human MC(TC) development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.