ArticleInfection and immunity2000
alpha(v)beta(3) integrin and bacterial lipopolysaccharide are involved in Coxiella burnetii-stimulated production of tumor necrosis factor by human monocytes.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 59 citations in OpenAlex.
- Q Fever Vaccines: Unveiling the Historical Journey and Contemporary Innovations in Vaccine Development.Vaccines · 2025Review
- Article
- Review
- To die or not to die: Programmed cell death responses and their interactions with Coxiella burnetii infection.Molecular microbiology · 2022Article
- The interplay of thyroid hormones and the immune system - where we stand and why we need to know about it.European journal of endocrinology · 2022Review
- Inhibition by Thyroid Hormones of Cell Migration Activated by IGF-1 and MCP-1 in THP-1 Monocytes: Focus on Signal Transduction Events Proximal to Integrin αvβ3.Frontiers in cell and developmental biology · 2021Article
- Recent Advances on the Innate Immune Response toFrontiers in cellular and infection microbiology · 2021Review
- TheFrontiers in cellular and infection microbiology · 2020Article
- "Hairiness" is a Facsimile of Reorganized Cytoskeletons: A Cytopathic Effect ofYonsei medical journal · 2019Review
- Article
- Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii.Veterinary research · 2017Article
- Coxiella burnetii Infects Primary Bovine Macrophages and Limits Their Host Cell Response.Infection and immunity · 2016Article
- Contrasting Lifestyles Within the Host Cell.Microbiology spectrum · 2016Review
- Coxiella burnetii Phagocytosis Is Regulated by GTPases of the Rho Family and the RhoA Effectors mDia1 and ROCK.PloS one · 2015Article
- Molecular pathogenesis of the obligate intracellular bacterium Coxiella burnetii.Nature reviews. Microbiology · 2013Review
- Tripping on acid: trans-kingdom perspectives on biological acids in immunity and pathogenesis.PLoS pathogens · 2013Review
- Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells.Immunology · 2009Article
- Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways.Respiratory research · 2009Article
- Antimicrobial peptides as new recognition molecules for screening challenging species.Sensors and actuators. B, Chemical · 2007Article
- Physicochemical characterization of the endotoxins from Coxiella burnetii strain Priscilla in relation to their bioactivities.BMC biochemistry · 2004Article
Corrections and comments
- Expression of concern
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coxiella burnetii, the agent of Q fever, enters human monocytes through alpha(v)beta(3) integrin and survives inside host cells. In addition, C. burnetii stimulates the synthesis of inflammatory cytokines including tumor necrosis factor (TNF) by monocytes. We studied the role of the interaction of C. burnetii with THP-1 monocytes in TNF production. TNF transcripts and TNF release reached maximum values within 4 h. Almost all monocytes bound C. burnetii after 4 h, while the percentage of phagocytosing monocytes did not exceed 20%. Cytochalasin D, which prevented the uptake of C. burnetii without interfering with its binding, did not affect the expression of TNF mRNA. Thus, bacterial adherence, but not phagocytosis, is necessary for TNF production by monocytes. The monocyte alpha(v)beta(3) integrin was involved in TNF synthesis since peptides containing RGD sequences and blocking antibodies against alpha(v)beta(3) integrin inhibited TNF transcripts induced by C. burnetii. Nevertheless, the cross-linking of alpha(v)beta(3) integrin by specific antibodies was not sufficient to induce TNF synthesis. The signal delivered by C. burnetii was triggered by bacterial lipopolysaccharide (LPS). Polymyxin B inhibited the TNF production stimulated by C. burnetii, and soluble LPS isolated from C. burnetii largely mimicked viable bacteria. On the other hand, avirulent variants of C. burnetii induced TNF production through an increased binding to monocytes rather than through the potency of their LPS. We suggest that the adherence of C. burnetii to monocytes via alpha(v)beta(3) integrin enables surface LPS to stimulate TNF production in THP-1 monocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.