ArticleThe Journal of physiology2000
Structural elements near the C-terminus are responsible for changes in nicotinic receptor gating kinetics following patch excision.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Roles of nicotinic acetylcholine receptor β subunit cytoplasmic loops in acute desensitization and single-channel features.Neuroscience · 2015Article
- Engineering a prokaryotic Cys-loop receptor with a third functional domain.The Journal of biological chemistry · 2011Article
- Pentobarbital produces activation and block of {alpha}1{beta}2{gamma}2S GABAA receptors in rapidly perfused whole cells and membrane patches: divergent results can be explained by pharmacokinetics.The Journal of general physiology · 2009Article
- Activation and block of the adult muscle-type nicotinic receptor by physostigmine: single-channel studies.Molecular pharmacology · 2008Article
- Activation of heteroliganded mouse muscle nicotinic receptors.The Journal of physiology · 2005Article
- Galantamine activates muscle-type nicotinic acetylcholine receptors without binding to the acetylcholine-binding site.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- The alpha 1 and alpha 6 subunit subtypes of the mammalian GABA(A) receptor confer distinct channel gating kinetics.The Journal of physiology · 2004Article
- Activation and block of mouse muscle-type nicotinic receptors by tetraethylammonium.The Journal of physiology · 2003Article
- Properties of the human muscle nicotinic receptor, and of the slow-channel myasthenic syndrome mutant epsilonL221F, inferred from maximum likelihood fits.The Journal of physiology · 2003Article
- The quality of maximum likelihood estimates of ion channel rate constants.The Journal of physiology · 2003Article
- Contributions of the non-alpha subunit residues (loop D) to agonist binding and channel gating in the muscle nicotinic acetylcholine receptor.The Journal of physiology · 2002Article
- Aromatics at the murine nicotinic receptor agonist binding site: mutational analysis of the alphaY93 and alphaW149 residues.The Journal of physiology · 2001Article
- How to force conformity on transmitter-gated channels.The Journal of general physiology · 2000Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have studied the effect of patch excision on the gating kinetics of muscle nicotinic acetylcholine receptors transiently expressed in HEK 293 cells. The experiments were performed on embryonic and adult wild-type, and several mutated, receptors using acetylcholine, carbamylcholine and tetramethylammonium as agonists. We show that patch excision of cell-attached patches into the inside-out configuration led to a reduction of mean open duration in receptors containing a gamma-subunit (embryonic) but not an epsilon-subunit (adult receptors). Kinetic analysis of an embryonic receptor containing a mutated residue, alphaY93F, showed that the reduction in the mean open duration upon patch excision was mainly caused by an increase in the channel closing rate constant. This was confirmed by experiments on embryonic wild-type receptors using carbamylcholine as an agonist with low efficacy. By expressing receptors containing chimeric gamma-epsilon subunits we found that segments of the gamma-subunit corresponding to a region within the M3-M4 linker (the amphipathic helix, HA) and the M4 transmembrane domain were required for the reduction in channel open duration after excision. The results indicate that particular residues in both M4 and HA are required to allow the change in open time after excision. This finding suggests that there is an interaction between these two regions in determining the modulation of gating kinetics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.