ArticleThe EMBO journal2000
An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 97 citations in OpenAlex.
- Massive intein content inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Calcimycin InhibitsACS infectious diseases · 2022Article
- SufB intein splicing in Mycobacterium tuberculosis is influenced by two remote conserved N-extein histidines.Bioscience reports · 2022Article
- Protein Splicing of Inteins: A Powerful Tool in Synthetic Biology.Frontiers in bioengineering and biotechnology · 2022Review
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms.International journal of molecular sciences · 2020Article
- Article
- Chemoenzymatic Semisynthesis of Proteins.Chemical reviews · 2020Review
- An expanded library of orthogonal split inteins enables modular multi-peptide assemblies.Nature communications · 2020Article
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- Approach control. Stereoelectronic origin of geometric constraints on N-to-S and N-to-O acyl shifts in peptides.Chemical science · 2018Article
- Sequential formation of two branched intermediates during protein splicing of class three inteins.Extremophiles : life under extreme conditions · 2017Article
- Article
- Intein Clustering Suggests Functional Importance in Different Domains of Life.Molecular biology and evolution · 2016Article
- An Unprecedented Combination of Serine and Cysteine Nucleophiles in a Split Intein with an Atypical Split Site.The Journal of biological chemistry · 2015Article
- Thiol-based redox switches.Biochimica et biophysica acta · 2014Review
- Structural and dynamical features of inteins and implications on protein splicing.The Journal of biological chemistry · 2014Review
- Protein splicing: how inteins escape from precursor proteins.The Journal of biological chemistry · 2014Review
- Inteins: Nature's Gift to Protein Chemists.Chemical science · 2014Article
- Structural and biochemical characterization of an active arylamine N-acetyltransferase possessing a non-canonical Cys-His-Glu catalytic triad.The Journal of biological chemistry · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Variations in the intein-mediated protein splicing mechanism are becoming more apparent as polymorphisms in conserved catalytic residues are identified. The conserved Ser or Cys at the intein N-terminus and the conserved intein penultimate His are absent in the KlbA family of inteins. These inteins were predicted to be inactive, since an N-terminal Ala cannot perform the initial reaction of the standard protein splicing pathway to yield the requisite N-terminal splice junction (thio)ester. Despite the presence of an N-terminal Ala and a penultimate Ser, the KlbA inteins splice efficiently using an alternative protein splicing mechanism. In this non-canonical pathway, the C-extein nucleophile attacks a peptide bond at the N-terminal splice junction rather than a (thio)ester bond, alleviating the need to form the initial (thio)ester at the N-terminal splice junction. The remainder of the two pathways is the same: branch resolution by Asn cyclization is followed by an acyl rearrangement to form a native peptide bond between the ligated exteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.