ArticleThe EMBO journal2000
beta-subunits of Snf1 kinase are required for kinase function and substrate definition.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 163 citations in OpenAlex.
- Phosphorylation of Golgin Imh1 by AMPK/Snf1 compromises Golgi compartmentalization by releasing Arl1-Imh1 axis.Molecular biology of the cell · 2025Article
- The Yeast Gsk-3 Kinase Mck1 Is Necessary for Cell Wall Remodeling in Glucose-Starved and Cell Wall-Stressed Cells.International journal of molecular sciences · 2025Article
- Article
- Systems level analysis of time and stimuli specific signaling through PKA.Molecular biology of the cell · 2024Article
- Chalkiness and premature controlled by energy homeostasis in OsNAC02 Ko-mutant during vegetative endosperm development.BMC plant biology · 2024Article
- Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches.Microbial cell (Graz, Austria) · 2024Article
- Nutrient-dependent signaling pathways that control autophagy in yeast.FEBS letters · 2024Review
- Advances inJournal of fungi (Basel, Switzerland) · 2023Review
- Regulation and role of CAMKK2 in prostate cancer.Nature reviews. Urology · 2022Review
- Snf1 Kinase Differentially RegulatesMicroorganisms · 2022Article
- A Suppressor Mutation in the β-Subunit Kis1 Restores Functionality of the SNF1 Complex inmSphere · 2021Article
- Drug resistance in diploid yeast is acquired through dominant alleles, haploinsufficiency, gene duplication and aneuploidy.PLoS genetics · 2021Article
- An AMP-activated protein kinase complex with two distinctive alpha subunits is involved in nutritional stress responses in Trypanosoma cruzi.PLoS neglected tropical diseases · 2021Article
- Characterization of3 Biotech · 2021Article
- Article
- Mig1 localization exhibits biphasic behavior which is controlled by both metabolic and regulatory roles of the sugar kinases.Molecular genetics and genomics : MGG · 2020Article
- Enhanced multi-stress tolerance and glucose utilization of Saccharomyces cerevisiae by overexpression of the SNF1 gene and varied beta isoform of Snf1 dominates in stresses.Microbial cell factories · 2020Article
- Altered metabolic regulation owing to gsp1 mutations encoding the nuclear small G protein in Saccharomyces cerevisiae.Current genetics · 2020Article
- A Snf1-related nutrient-responsive kinase antagonizes endocytosis in yeast.PLoS genetics · 2020Article
- Fine-Tuning of Energy Levels RegulatesFrontiers in physiology · 2020Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Snf1 kinase and its mammalian homolog, the AMP-activated protein kinase, are heterotrimeric enzymes composed of a catalytic alpha-subunit, a regulatory gamma-subunit and a beta-subunit that mediates heterotrimer formation. Saccharomyces cerevisiae encodes three beta-subunit genes, SIP1, SIP2 and GAL83. Earlier studies suggested that these subunits may not be required for Snf1 kinase function. We show here that complete and precise deletion of all three beta-subunit genes inactivates the Snf1 kinase. The sip1Delta sip2Delta gal83Delta strain is unable to derepress invertase, grows poorly on alternative carbon sources and fails to direct the phosphorylation of the Mig1 and Sip4 proteins in vivo. The SIP1 sip2Delta gal83Delta strain manifests a subset of Snf phenotypes (Raf(+), Gly(-)) observed in the snf1Delta 10 strain (Raf(-), Gly(-)), suggesting that individual beta-subunits direct the Snf1 kinase to a subset of its targets in vivo. Indeed, deletion of individual beta-subunit genes causes distinct differences in the induction and phosphorylation of Sip4, strongly suggesting that the beta-subunits play an important role in substrate definition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.