ArticleMolecular biotechnology2000
Adaptation of mammalian cells to growth in serum-free media.
Article in Molecular biotechnology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Transcriptomic Insights Into Serum-Free Medium Adaptation and Temperature Reduction in Chinese Hamster Ovary Cell Cultures.Biotechnology journal · 2025Article
- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Advancing humanized 3D tumor modeling using an open access xeno-free medium.Frontiers in toxicology · 2025Article
- Animal Cell Lines as Expression Platforms in Viral Vaccine Production: A Post Covid-19 Perspective.ACS omega · 2024Review
- Efficient production of recombinant proteins in suspension CHO cells culture using the Tol2 transposon system coupled with cycloheximide resistance selection.Scientific reports · 2023Article
- Vero cells gain renal tubule markers in low-calcium and magnesium chemically defined media.Scientific reports · 2022Article
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
- Mammalian Cell Culture as a Platform for Veterinary Vaccines.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Establishment of a Suspension MDBK Cell Line in Serum-Free Medium for Production of Bovine Alphaherpesvirus-1.Vaccines · 2021Article
- Platforms for Production of Protein-Based Vaccines: From Classical to Next-Generation Strategies.Biomolecules · 2021Review
- Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.PloS one · 2020Article
- Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins.Cytotechnology · 2019Article
- Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody.Scientific reports · 2018Article
- Importance of Interaction between Integrin and Actin Cytoskeleton in Suspension Adaptation of CHO cells.Applied biochemistry and biotechnology · 2016Article
- Morphological characterization of cells in concentrated suspensions using multispectral diffuse optical tomography.Optics communications · 2012Article
- Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells.Molecular biotechnology · 2008Article
- Cells by design: a mini-review of targeting cell engineering using DNA microarrays.Molecular biotechnology · 2008Review
- Anoikis-resistant MDCK cells carrying susceptibilities to TNF-alpha and verotoxin that are suitable for influenza virus cultivation.Cytotechnology · 2006Article
- Production of recombinant protein therapeutics in cultivated mammalian cells.Nature biotechnology · 2004Review
- Transcriptomic functional characterization of recombinant adeno-associated virus producing cell line adapted to suspension-growth.Biotechnology progressArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A three-step protocol is described for adapting an anchorage-dependent, serum-dependent recombinant mammalian cell lineage to high density serum-free suspension culture. The objective is a cell lineage that is well-suited for the manufacture of a recombinant protein. The first step of the protocol generates an anchorage-independent cell lineage by culturing trypsin-treated cells in spinner flasks using serum-containing medium. The second step adapts the lineage to serum-free medium through a series of serum reduction steps in the presence of defined growth-promoting additives. The third step adapts the lineage to high-cell-density conditions by culturing the cells in a bioreactor in a manner that allows development of tolerance to growth-inhibiting substances released by the cells. Examples are presented for the use of this protocol for recombinant CHO cells.
Indexed as
Identifiers
10986701What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.