ArticleJournal of bacteriology2000
Molecular analysis of sucrose metabolism of Erwinia amylovora and influence on bacterial virulence.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
- Beneath the Bark: Molecular Determinants of Adaptation and Infection in Bacterial Pathogens of Woody Hosts.Molecular plant pathology · 2026Review
- Whole-genome characterization and analysis of Pantoea agglomerans R6: a genomic insight into its pathogenicity and resistance as a potential opportunistic plant pathogen.BMC genomics · 2026Article
- A critical role ofPNAS nexus · 2026Article
- A Sucrose-Utilisation Gene Cluster Contributes to Colonisation of Horse Chestnut by Pseudomonas syringae pv. aesculi.Molecular plant pathology · 2025Article
- Review
- The Scr and Csc pathways for sucrose utilization co-exist inFrontiers in microbiology · 2024Article
- Untargeted metabolomics coupled with genomics in the study of sucrose and xylose metabolism inFrontiers in microbiology · 2024Article
- Comparison of Bacterial Community of Healthy and Erwinia amylovora Infected Apples.The plant pathology journal · 2021Article
- Identification of an Invertase With High Specific Activity for Raffinose Hydrolysis and Its Application in Soymilk Treatment.Frontiers in microbiology · 2021Article
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- Genome analysis of two novel Pseudomonas strains exhibiting differential hypersensitivity reactions on tobacco seedlings reveals differences in nonflagellar T3SS organization and predicted effector proteins.MicrobiologyOpen · 2018Article
- Conservation of Erwinia amylovora pathogenicity-relevant genes among Erwinia genomes.Archives of microbiology · 2017Article
- Comparative transcriptome analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars - susceptible and resistant to fire blight.BMC genomics · 2017Article
- Virulence Factors of Erwinia amylovora: A Review.International journal of molecular sciences · 2015Review
- Small-molecule inhibitors suppress the expression of both type III secretion and amylovoran biosynthesis genes in Erwinia amylovora.Molecular plant pathology · 2014Article
- Discovery of plant phenolic compounds that act as type III secretion system inhibitors or inducers of the fire blight pathogen, Erwinia amylovora.Applied and environmental microbiology · 2013Article
- Article
- Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae.BMC genomics · 2010Article
- Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sucrose is an important storage and transport sugar of plants and an energy source for many phytopathogenic bacteria. To analyze regulation and biochemistry of sucrose metabolism of the fire blight pathogen Erwinia amylovora, a chromosomal fragment which enabled Escherichia coli to utilize sucrose as sole carbon source was cloned. By transposon mutagenesis, the scr regulon of E. amylovora was tagged, and its nucleotide sequence was determined. Five open reading frames, with the genes scrK, scrY, scrA, scrB, and scrR, had high homology to genes of the scr regulons from Klebsiella pneumoniae and plasmid pUR400. scrB and scrR of E. amylovora were fused to a histidine tag and to the maltose-binding protein (MalE) of E. coli, respectively. ScrB (53 kDa) catalyzed the hydrolysis of sucrose with a K(m) of 125 mM. Binding of a MalE-ScrR fusion protein to an scrYAB promoter fragment was shown by gel mobility shifts. This complex dissociated in the presence of fructose but not after addition of sucrose. Expression of the scr regulon was studied with an scrYAB promoter-green fluorescent protein gene fusion and measured by flow cytometry and spectrofluorometry. The operon was affected by catabolite repression and induced by sucrose or fructose. The level of gene induction correlated to the sucrose concentration in plant tissue, as shown by flow cytometry. Sucrose mutants created by site-directed mutagenesis did not produce significant fire blight symptoms on apple seedlings, indicating the importance of sucrose metabolism for colonization of host plants by E. amylovora.
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