Evidence map›Paper›PMID 10983236›Full record

ArticleFolia microbiologica1999

Development of the primer set for the detection of the hsp60 gene in Trichophyton mentagrophytes cDNA.

P Kopecek, M Raska, E Weigl

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Folia microbiologica, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

P KopecekDepartment of Biology, Medical Faculty, Palacký University, Olomouc, Czech Republic. kopecek@tunw.upol.cz
M Raska
E Weigl
Palacký University Olomouc · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three sequences of hsp60 from Saccharomyces cerevisiae, Schizosaccharomyces pombe and Histoplasma capsulatum were compared. Local multiple alignment of these sequences allowed the selection of two oligonucleotides suitable as primers for the polymerase chain reaction. This primer set was used for the amplification of a part of the hsp60 gene from cDNA of Trichophyton mentagrophytes and S. cerevisiae. Similar fragments detected in both PCR's imply the possible future use of the developed primer set for the detection of the hsp60 gene in other fungal species.

Indexed as

Genes, FungalBase SequenceChaperonin 60DNA, ComplementaryDNA, FungalDNA PrimersHistoplasmaReverse Transcriptase Polymerase Chain ReactionSaccharomyces cerevisiaeSchizosaccharomycesSequence Homology, Nucleic AcidSpecies SpecificityTrichophytonChaperonin 60DNA, ComplementaryDNA, FungalDNA Primers

Identifiers

PMID10983236
OpenAlexW2013651481

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.