ArticleNucleic acids research2000
Psoralen photo-crosslinked mRNA-puromycin conjugates: a novel template for the rapid and facile preparation of mRNA-protein fusions.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Improvement of cDNA TRAP Display via Optimization of Puromycin Linker Design for Enhanced Discovery of Antibody-Like Proteins.Chembiochem : a European journal of chemical biology · 2026Article
- Acceleration of the Deamination of Cytosine through Photo-Crosslinking.Current issues in molecular biology · 2023Article
- Development of a Cyclic, Cell Penetrating Peptide Compatible with In Vitro Selection Strategies.ACS chemical biology · 2023Article
- Cell-Free Display Techniques for Protein Evolution.Advances in biochemical engineering/biotechnology · 2023Article
- DNA Tiling Enables Precise Acylation-Based Labeling and Control of mRNA.Angewandte Chemie (International ed. in English) · 2021Article
- Directing evolution of novel ligands by mRNA display.Chemical Society reviews · 2021Review
- Resources and Methods for Engineering "Designer" Glycan-Binding Proteins.Molecules (Basel, Switzerland) · 2021Review
- VISTA is an acidic pH-selective ligand for PSGL-1.Nature · 2019Article
- A Novel gp41-Binding Adnectin with Potent Anti-HIV Activity Is Highly Synergistic when Linked to a CD4-Binding Adnectin.Journal of virology · 2018Article
- Adnectin-drug conjugates for Glypican-3-specific delivery of a cytotoxic payload to tumors.Protein engineering, design & selection : PEDS · 2018Article
- Study of Photochemical Cytosine to Uracil Transition via Ultrafast Photo-Cross-Linking Using Vinylcarbazole Derivatives in Duplex DNA.Molecules (Basel, Switzerland) · 2018Article
- Discovery of a 29-Amino-Acid Reactive Abiotic Peptide for Selective Cysteine Arylation.ACS chemical biology · 2018Article
- Rapid Capture and Release of Nucleic Acids through a Reversible Photo-Cycloaddition Reaction in a Psoralen-Functionalized Hydrogel.Angewandte Chemie (International ed. in English) · 2018Article
- Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity.Antimicrobial agents and chemotherapy · 2017Article
- Extensive libraries of gene truncation variants generated by in vitro transposition.Nucleic acids research · 2017Article
- Highly Constrained Bicyclic Scaffolds for the Discovery of Protease-Stable Peptides via mRNA Display.ACS chemical biology · 2017Article
- Directed Evolution of Glycopeptides Using mRNA Display.Methods in enzymology · 2017Article
- Directed evolution of multivalent glycopeptides tightly recognized by HIV antibody 2G12.Journal of the American Chemical Society · 2014Article
- Universal strategies for the DNA-encoding of libraries of small molecules using the chemical ligation of oligonucleotide tags.Artificial DNA, PNA & XNA · 2014Article
- Increasing the library size in cDNA display by optimizing purification procedures.Biological procedures online · 2013Article
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3 authors.
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Abstract
We describe the synthesis of a novel type of mRNA template and its use in the preparation of mRNA-protein fusions. A light-induced psoralen crosslinking reaction was used to attach a puromycin-containing oligonucleotide to the 3'-end of an mRNA template. The photo-crosslinked template was found to undergo efficient mRNA-protein fusion formation in rabbit reticulocyte lysate. Fusion formation was subsequently tested with templates carrying puromycin linkers of different length and chemical composition. Short linkers with multiple triethyleneglycol phosphate building blocks allowed the most efficient fusion formation under a wide range of salt conditions. The present method simplifies the preparation of mRNA-protein fusions and thus significantly accelerates the in vitro protein evolution procedure which involves repetitive cycles of fusion production and selection.
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