ArticleNucleic acids research2000
Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- The lariat RNA debranching enzyme DBR1 enhances HIF-1 signaling and chemoresistance at the expense of lariat RNA processing.The Journal of biological chemistry · 2026Article
- Microcargos with Megaeffects: A Literature Review of Immunomodulatory, Diagnostic, Prognostic, and Therapeutic Dimensions of Exosomal MicroRNAs in Glioblastoma Multiforme.Cellular and molecular neurobiology · 2025Review
- DBR1 orchestrates the fate of lariat RNA: debranching-dependent turnover and function.Nucleic acids research · 2025Review
- Human DBR1 deficiency impairs stress granule-dependent PKR antiviral immunity.The Journal of experimental medicine · 2025Article
- A repertoire of intronic lariat RNAs reveals tissue-specific regulation and target mimicry potential in plants.Science China. Life sciences · 2024Article
- A founder DBR1 variant causes a lethal form of congenital ichthyosis.Human genetics · 2023Article
- Maturation of small nucleolar RNAs: from production to function.RNA biology · 2023Review
- Intron turnover is essential to the development and pathogenicity of the plant pathogenic fungus Fusarium graminearum.Communications biology · 2022Article
- SICKLE modulates lateral root development by promoting degradation of lariat intronic RNA.Plant physiology · 2022Article
- A Comprehensive Map of Intron Branchpoints and Lariat RNAs in Plants.The Plant cell · 2019Article
- ShapeShifter: a novel approach for identifying and quantifying stable lariat intronic species in RNAseq data.Quantitative biology (Beijing, China) · 2018Article
- Article
- Unexplored Molecular Features of theFrontiers in cellular and infection microbiology · 2018Article
- Metal dependence and branched RNA cocrystal structures of the RNA lariat debranching enzyme Dbr1.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis.PLoS genetics · 2016Article
- In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity.Scientific reports · 2016Article
- Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.International journal of molecular sciences · 2015Article
- ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae.PloS one · 2015Article
- Genome wide in silico analysis of Plasmodium falciparum phosphatome.BMC genomics · 2014Article
- LaSSO, a strategy for genome-wide mapping of intronic lariats and branch points using RNA-seq.Genome research · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA encoding the human RNA lariat debranching enzyme (hDBR1) was identified and cloned by searching the Expressed Sequence Tag (EST) database and screening a HeLa cDNA library, based on predicted amino acid sequence homologies with the Saccharomyces cerevisiae, Schizosaccharomyces pombe and Caenorhabditis elegans debranching enzymes. The hDBR1 cDNA expressed in Escherichia coli showed debranching activity in vitro and was also shown to be functional in an interspecies specific complementation experiment. hDBR1 cDNA in a S. cerevisiae expression vector complemented the intron accumulation phenotype of a S. cerevisiae dbr1 null mutant. Integration of the cDNA for hDBR1 into the ura4 locus of S. pombe also complemented both the intron accumulation and slow growth phenotypes of a S. pombe dbr1 null mutant strain. Comparison of the amino acid sequence of hDBR1 with the other DBR protein sequences showed several conserved regions, with 40, 44 and 43% identity to the S. cerevisiae, S. pombe and C. elegans debranching enzymes, respectively.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.