ArticleNucleic acids research2000
Parallel intramolecular DNA triple helix with G and T bases in the third strand stabilized by Zn(2+) ions.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Structure and stability of different triplets involving artificial nucleobases: clues for the formation of semisynthetic triple helical DNA.Scientific reports · 2023Article
- LncRNA Hmrhl regulates expression of cancer related genes in chronic myelogenous leukemia through chromatin association.NAR cancer · 2021Article
- (C2G4)n repeat expansion sequences from the C9orf72 gene form an unusual DNA higher-order structure in the pH range of 5-6.PloS one · 2018Article
- Intrastrand triplex DNA repeats in bacteria: a source of genomic instability.Nucleic acids research · 2015Article
- MEG3 long noncoding RNA regulates the TGF-β pathway genes through formation of RNA-DNA triplex structures.Nature communications · 2015Article
- Structural properties of g,t-parallel duplexes.Journal of nucleic acids · 2010Article
- Evaluation of effects of bivalent cations on the formation of purine-rich triple-helix DNA by ESI-FT-MS.Journal of the American Society for Mass Spectrometry · 2009Article
- Circular dichroism and conformational polymorphism of DNA.Nucleic acids research · 2009Review
- Interarm interaction of DNA cruciform forming at a short inverted repeat sequence.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present evidence of formation of an intramolecular parallel triple helix with T*A.T and G*G.C base triplets (where * represents the hydrogen bonding interaction between the third strand and the duplex while. represents the Watson-Crick interactions which stabilize the duplex). The third GT strand, containing seven GpT/TpG steps, targets the polypurine sequence 5'-AGG-AGG-GAG-GAG-3'. The triple helix is obtained by the folding back twice of a 36mer, formed by three dodecamers tethered by hydroxyalkyl linkers (-L-). Due to the design of the oligonucleotide, the third strand orientation is parallel with respect to the polypurine strand. Triple helical formation has been studied in concentration conditions in which native gel electrophoresis experiments showed the absence of intermolecular structures. Circular dichroism (CD) and UV spectroscopy have been used to evidence the triplex structure. A CD spectrum characteristic of triple helical formation as well as biphasic UV and CD melting curves have been obtained in high ionic strength NaCl solutions in the presence of Zn(2+) ions. Specific interactions with Zn(2+) ions in low water activity conditions are necessary to stabilize the parallel triplex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.