ArticlePlant physiology2000
Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 132 citations in OpenAlex.
- High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor inPlant biotechnology (Tokyo, Japan) · 2022Article
- Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein inPlants (Basel, Switzerland) · 2021Article
- Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells.International journal of molecular sciences · 2021Article
- The M4 insulator, the TM2 matrix attachment region, and the double copy of the heavy chain gene contribute to the enhanced accumulation of the PHB-01 antibody in tobacco plants.Transgenic research · 2020Article
- The Effect of Methyl Jasmonate and Temperature on the Transient Expression of Recombinant Proteins in Cucurbita pepo L.Molecular biotechnology · 2019Article
- Production of Biopharmaceuticals inFrontiers in plant science · 2019Article
- Article
- Induction of a dwarf phenotype with IBH1 may enable increased production of plant-made pharmaceuticals in plant factory conditions.Plant biotechnology journal · 2016Article
- High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures.Plant biotechnology journal · 2016Article
- Tackling Unwanted Proteolysis in Plant Production Hosts Used for Molecular Farming.Frontiers in plant science · 2016Review
- Identification, Characterization and Down-Regulation of Cysteine Protease Genes in Tobacco for Use in Recombinant Protein Production.PloS one · 2015Article
- Improved expression of recombinant plant-made hEGF.Plant cell reports · 2014Article
- Antimalarial efficacy, cytotoxicity, and genotoxicity of methanolic stem bark extract from Hintonia latiflora in a Plasmodium yoelii yoelii lethal murine malaria model.Parasitology research · 2014Article
- The human anti-HIV antibodies 2F5, 2G12, and PG9 differ in their susceptibility to proteolytic degradation: down-regulation of endogenous serine and cysteine proteinase activities could improve antibody production in plant-based expression platforms.Biotechnology journal · 2014Article
- Effect of the developmental stage and tissue position on the expression and glycosylation of recombinant glycoprotein GA733-FcK in transgenic plants.Frontiers in plant science · 2014Article
- Protection of recombinant mammalian antibodies from development-dependent proteolysis in leaves of Nicotiana benthamiana.PloS one · 2013Article
- Recombinant monoclonal antibody yield in transgenic tobacco plants is affected by the wounding response via an ethylene dependent mechanism.Transgenic research · 2012Article
- Adsorptive loss of secreted recombinant proteins in transgenic rice cell suspension cultures.Plant cell reports · 2012Article
- Antibody degradation in tobacco plants: a predominantly apoplastic process.BMC biotechnology · 2011Article
- Tobacco as biofactory for biologically active hPL production: a human hormone with potential applications in type-1 diabetes.Transgenic research · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants are regarded as a promising system for the production of heterologous proteins. However, little is known about the influence of plant physiology and plant development on the yield and quality of the heterologous proteins produced in plants. To investigate this, tobacco (Nicotiana tabacum cv Samsun NN) was transformed with a single construct that contained behind constitutive promotors the light- and heavy-chain genes of a mouse antibody. The in planta stability of the antibody was analyzed in transgenic plants that were grown under high and low irradiation at 15 degrees C and 25 degrees C. High-light conditions favored the production of biomass, of total soluble protein, and of antibody. The plants grown at 25 degrees C developed faster and contained less antibody per amount of leaf tissue than the plants grown at 15 degrees C. Both endogenous protein and antibody content showed a strong decline during leaf development. The heavy chains of the antibody underwent in planta degradation via relatively stable fragments. In vitro incubations of purified plantibody with leaf extracts of wild-type tobacco indicated the involvement of acidic proteases. It is interesting that the same antibody produced by mouse hybridoma cells exhibited higher stability in this in vitro assay. This may be explained by the assumption that the plant type of N-glycosylation contributes less to the stability of the antibody than the mouse-type of N-glycosylation. The results of this study indicate that proteolytic degradation during plant development can be an important factor affecting yield and homogeneity of heterologous protein produced by transgenic plants.
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