ArticleMolecular biology of the cell2000
PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin alphavbeta3 in Cis.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 108 citations in OpenAlex.
- Article
- Atomic Level Dissection of the Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1) Homophilic Binding Interface: Implications for Endothelial Cell Barrier Function.Arteriosclerosis, thrombosis, and vascular biology · 2022Article
- Microfluidic device engineered to study the trafficking of multiple myeloma cancer cells through the sinusoidal niche of bone marrow.Scientific reports · 2022Article
- Appearance of claudin-5Journal of neuroinflammation · 2021Article
- Development and Application of Endothelial Cells Derived From Pluripotent Stem Cells in Microphysiological Systems Models.Frontiers in cardiovascular medicine · 2021Review
- Cancer discrimination by on-cell N-glycan ligation.Communications chemistry · 2020Article
- New Interfaces of Thyroid Hormone Actions With Blood Coagulation and Thrombosis.Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis · 2018Review
- Homotypic endothelial nanotubes induced by wheat germ agglutinin and thrombin.Scientific reports · 2018Article
- CRISPR-mediated deletion of the PECAM-1 cytoplasmic domain increases receptor lateral mobility and strengthens endothelial cell junctional integrity.Life sciences · 2018Article
- In Situ Ligation of High- and Low-Affinity Ligands to Cell Surface Receptors Enables Highly Selective Recognition.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Structural basis for PECAM-1 homophilic binding.Blood · 2016Article
- Cell surface and in vivo interaction of dendrimeric N-glycoclusters.Glycoconjugate journal · 2015Review
- PECAM-1 isoforms, eNOS and endoglin axis in regulation of angiogenesis.Clinical science (London, England : 1979) · 2015Review
- Statins as Pleiotropic Modifiers of Vascular Oxidative Stress and Inflammation.Journal of critical care medicine (Universitatea de Medicina si Farmacie din Targu-Mures) · 2015Review
- T11TS inhibits glioma angiogenesis by modulation of MMPs, TIMPs, with related integrin αv and TGF-β1 expressions.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Proteinase 3 contributes to transendothelial migration of NB1-positive neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Role of PECAM-1 in arteriogenesis and specification of preexisting collaterals.Circulation research · 2010Article
- TCR stimulation drives cleavage and shedding of the ITIM receptor CD31.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Sphingosine kinase-1 associates with integrin {alpha}V{beta}3 to mediate endothelial cell survival.The American journal of pathology · 2009Article
- Endothelial cell PECAM-1 promotes atherosclerotic lesions in areas of disturbed flow in ApoE-deficient mice.Arteriosclerosis, thrombosis, and vascular biology · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PECAM-1/CD31 is a cell adhesion and signaling molecule that is enriched at the endothelial cell junctions. Alternative splicing generates multiple PECAM-1 splice variants, which differ in their cytoplasmic domains. It has been suggested that the extracellular ligand-binding property, homophilic versus heterophilic, of these isoforms is controlled by their cytoplasmic tails. To determine whether the cytoplasmic domains also regulate the cell surface distribution of PECAM-1 splice variants, we examined the distribution of CD31-EGFPs (PECAM-1 isoforms tagged with the enhanced green fluorescent protein) in living Chinese hamster ovary cells and in PECAM-1-deficient endothelial cells. Our results indicate that the extracellular, rather than the cytoplasmic domain, directs PECAM-1 to the cell-cell borders. Furthermore, coculturing PECAM-1 expressing and deficient cells along with transfection of CD31-EGFP cDNAs into PECAM-1 deficient cells reveal that this PECAM-1 localization is mediated by homophilic interactions. Although the integrin alphavbeta3 has been shown to interact with PECAM-1, this trans-heterophilic interaction was not detected at the borders of endothelial cells. However, based on cocapping experiments performed on proT cells, we provide evidence that the integrin alphavbeta3 associates with PECAM-1 on the same cell surface as in a cis manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.