ArticleMolecular biology of the cell2000
Intracellular redirection of plasma membrane trafficking after loss of epithelial cell polarity.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Bulk delivery of a preassembled apical surface initiates epithelial lumen formation.Nature communications · 2026Article
- Development of a cyst-targeted therapy for polycystic kidney disease using an antagonistic dimeric IgA monoclonal antibody against cMET.Cell reports. Medicine · 2025Article
- Lack of discreet colocalization of epithelial apoptosis to the atretic precursor in the colon of the Fibroblast growth factor receptor 2IIIb mouse and staining consistent with cellular movement suggest a revised model of atresia formation.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Soluble syntaxin 3 functions as a transcriptional regulator.The Journal of biological chemistry · 2018Article
- Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells.Bio-protocol · 2018Article
- Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.PloS one · 2015Article
- Disease-causing mutations associated with four bestrophinopathies exhibit disparate effects on the localization, but not the oligomerization, of Bestrophin-1.Experimental eye research · 2014Article
- Milk secretion: The role of SNARE proteins.Journal of mammary gland biology and neoplasia · 2014Review
- t-SNARE Syntaxin2 (STX2) is implicated in intracellular transport of sulfoglycolipids during meiotic prophase in mouse spermatogenesis.Biology of reproduction · 2013Article
- Adducins regulate remodeling of apical junctions in human epithelial cells.Molecular biology of the cell · 2010Article
- Phylogeny of endocytic components yields insight into the process of nonendosymbiotic organelle evolution.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Par1b promotes hepatic-type lumen polarity in Madin Darby canine kidney cells via myosin II- and E-cadherin-dependent signaling.Molecular biology of the cell · 2007Article
- Syntaxins 3 and 4 are concentrated in separate clusters on the plasma membrane before the establishment of cell polarity.Molecular biology of the cell · 2006Article
- Rab11a and myosin Vb are required for bile canalicular formation in WIF-B9 cells.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Mechanism of IFN-gamma-induced endocytosis of tight junction proteins: myosin II-dependent vacuolarization of the apical plasma membrane.Molecular biology of the cell · 2005Article
- Differential roles for actin polymerization and a myosin II motor in assembly of the epithelial apical junctional complex.Molecular biology of the cell · 2005Article
- Localization of plasma membrane t-SNAREs syntaxin 2 and 3 in intracellular compartments.BMC cell biology · 2005Article
- PATJ regulates tight junction formation and polarity in mammalian epithelial cells.The Journal of cell biology · 2005Article
- Par-1 promotes a hepatic mode of apical protein trafficking in MDCK cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In polarized Madin-Darby canine kidney epithelial cells, components of the plasma membrane fusion machinery, the t-SNAREs syntaxin 2, 3, and 4 and SNAP-23, are differentially localized at the apical and/or basolateral plasma membrane domains. Here we identify syntaxin 11 as a novel apical and basolateral plasma membrane t-SNARE. Surprisingly, all of these t-SNAREs redistribute to intracellular locations when Madin-Darby canine kidney cells lose their cellular polarity. Apical SNAREs relocalize to the previously characterized vacuolar apical compartment, whereas basolateral SNAREs redistribute to a novel organelle that appears to be the basolateral equivalent of the vacuolar apical compartment. Both intracellular plasma membrane compartments have an associated prominent actin cytoskeleton and receive membrane traffic from cognate apical or basolateral pathways, respectively. These findings demonstrate a fundamental shift in plasma membrane traffic toward intracellular compartments while protein sorting is preserved when epithelial cells lose their cell polarity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.