ArticleJournal of virology2000
Nitric oxide synthesis enhances human immunodeficiency virus replication in primary human macrophages.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Metabolic control of macrophages in coronavirus disease 2019.Virulence · 2026Review
- HIV Infection, Neurotoxicity, Inflammation, Premature Aging, and Therapeutic Challenges to PLWH: An Overview.International journal of molecular sciences · 2026Review
- Differences between human and rodent nitric oxide production dictate susceptibility to tick-bornebioRxiv : the preprint server for biology · 2025Article
- The Involvement of Neutrophil in the Immune Dysfunction Associated with BVDV Infection.Pathogens (Basel, Switzerland) · 2023Article
- Multi-omic comparative analysis of COVID-19 and bacterial sepsis-induced ARDS.PLoS pathogens · 2022Article
- Multi-omic comparative analysis of COVID-19 and bacterial sepsis-induced ARDS.medRxiv : the preprint server for health sciences · 2022Article
- Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis.eLife · 2021Article
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- The role of catecholamines in HIV neuropathogenesis.Brain research · 2019Review
- Nutritional supplementation with the mushroom Agaricus sylvaticus reduces oxidative stress in children with HIV.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- PknE, a serine/threonine protein kinase of Mycobacterium tuberculosis initiates survival crosstalk that also impacts HIV coinfection.PloS one · 2014Article
- The intracellular environment of human macrophages that produce nitric oxide promotes growth of mycobacteria.Infection and immunity · 2013Article
- Characterization of the placental macrophage secretome: implications for antiviral activity.Placenta · 2009Article
- Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy.Retrovirology · 2008Article
- Nitric oxide and peroxynitrite have different antiviral effects against hantavirus replication and free mature virions.European journal of immunology · 2006Article
- Reovirus infection of the CNS enhances iNOS expression in areas of virus-induced injury.Experimental neurology · 2005Article
- Nitric oxide inhibits the replication cycle of severe acute respiratory syndrome coronavirus.Journal of virology · 2005Article
- CCTTT-repeat polymorphism of the inducible nitric oxide synthase is not associated with HIV pathogenesis.Clinical and experimental immunology · 2004Article
- Astrovirus-induced synthesis of nitric oxide contributes to virus control during infection.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macrophages are suspected to play a major role in human immunodeficiency virus (HIV) infection pathogenesis, not only by their contribution to virus dissemination and persistence in the host but also through the dysregulation of immune functions. The production of NO, a highly reactive free radical, is thought to act as an important component of the host immune response in several viral infections. The aim of this study was to evaluate the effects of HIV type 1 (HIV-1) Ba-L replication on inducible nitric oxide synthase (iNOS) mRNA expression in primary cultures of human monocyte-derived macrophages (MDM) and then examine the effects of NO production on the level of HIV-1 replication. Significant induction of the iNOS gene was observed in cultured MDM concomitantly with the peak of virus replication. However, this induction was not accompanied by a measurable production of NO, suggesting a weak synthesis of NO. Surprisingly, exposure to low concentrations of a NO-generating compound (sodium nitroprusside) and L-arginine, the natural substrate of iNOS, results in a significant increase in HIV replication. Accordingly, reduction of L-arginine bioavailability after addition of arginase to the medium significantly reduced HIV replication. The specific involvement of NO was further demonstrated by a dose-dependent inhibition of viral replication that was observed in infected macrophages exposed to N(G)-monomethyl L-arginine and N(G)-nitro-L-arginine methyl ester (L-NAME), two inhibitors of the iNOS. Moreover, an excess of L-arginine reversed the addition of L-NAME, confirming that an arginine-dependent mechanism is involved. Finally, inhibitory effects of hemoglobin which can trap free NO in culture supernatants and in biological fluids in vivo confirmed that endogenously produced NO could interfere with HIV replication in human macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.